H.^ MATE IN loo 



weeks [of course, if you make it uj) Avith the ripened brown 

 alcoholic solution recommended § 277 sub fin., this will be super- 

 fluous]. Stain from twenty-four to thirty-six hours. Use the 

 same staining solution over and over again until it becomes spoilt ; 

 for the solution after having been used gives a more energetic 

 stain, owing to its containing a trace of iron brought over by the 

 sections. Differentiate in a 2| per cent, solution of ferric alum. 

 Rinse for ten minutes in running water, clear with xylol, not 

 with any essential oil, and mount in xylol-balsam. See also 

 under " Centrosomes," and " Chromosomes," etc. 



BiELASZEWics {Bull. Acud. Cracovie, 1909, 2 serie, p. 152) differentiates 

 with very weak solution of calcium chloride ; Guarnieri {Mon. Zool. 

 Ital., xvii, 1906, p. 44) with saturated solution of picric acid. 



GuRWiTScn {Zeit. xviss. Mik., xviii, 1902, p. 291) floods sections on 

 the slide with mordant, warms on a water-bath till bubbles are given off 

 or the mordant becomes turbid, then stains with the ha?niatoxyIin in 

 the same way. The whole process takes about ten minutes. 



Held {Arch. Anat. Phijs., Anat. Ahth., 1897, p. 277) adds to the 

 staining bath a very little of the iron-alum solution until a scarcely 

 perceptible precipitate is produced. A dangerous practice. Lee found 

 it is not even safe to add a little of an over-used solution {supra). 



Francotte {Arch. Zool. Exper., vi, 1898, p. 200) mordants with 

 tartrate of iron, Mallory {Journ. Exper. Med., v, 1900, p. 15) with 

 chloride. 



283. Iron Haematoxylin (Butschli, Unters. nber mikroskopische 

 Schsume u. das Protoplasma, etc., 1892, p. 80). Sections treated with a 

 weak brown aqueous solution of ferric acetate, washed with water, and 

 stained in 0-5 per cent, aqueous solution of haematoxylin. A stain of 

 extraordinary intensity, used by Biitsehli for sections, 1 [jl in thickness, 

 of Protozoa. 



284. Weigert's Iron Haematoxylin Mixture {Zeit. iviss. Mik., xxi, 

 1904, p. 1). Mix 1 part of a 1 per cent, solution of haematoxylin in 

 alcohol of 96 per cent, with 1 of a solution containing 4 c.c. of licj. Jerri 

 sesquichlor., 1 c.c. of officinal hydrochloric acid (sp. gr. 1124) and 95 

 of water. The mixture may be kept for some days (until it begins to 

 smell of ether), but is best used fresh. Stain sections for a few minutes ; 

 no differentiation is necessary. 



For an earlier process of Weigert's {Allg. Zeit. Psychiatr., 1894, 

 p. 245) see last edition. 



Morel and Bassal {Journ. Anat. Phys., xlv, 1909, p. 632) stain in 

 bulk in Weigert's mixture with the addition of 1 c.c. of 4 per cent, 

 solution of acetate of copper. 



285. Janssens' Iron Haematoxylin ( " Hematoxyline noire"; La 

 Cellule, xiv, 1897, p. 207). A similar mixture to that of Delafield, 

 ferric alum being taken instead of ammonia alum, the rest as in 

 Delafield's. A progressive stain, nuclear : for yeast cells. 



286. Hansen's Iron Haematoxylin {Zeit. wiss. Mik., xxii, 1905, p. 55). 

 A solution of 10 grm. ferric alum in 150 c.c. water is added to a solution 

 of 1-6 grm. haematoxylin in 75 c.c. water, the mixture heated to boiling- 

 point and cooled without access of air. Filter before use. To get a 

 pure nuclear stain, add dilute sulphuric acid. 



287. Aluminium Haematein (Alum Haematoxylin) Generalities. 



The mordant and dye are generally combined in a single staining 



