158 HMMATEIN 



the like, to make a double staining mixture ; but it seems pre- 

 ferable to use the solutions in succession. 



289. Mayer's Acid Haemalum {MiWi. Zool. Stat. Neapel, x, 

 1891, p. 174). This is ha?malum with 2 per cent, glacial acetic 

 acid (or 4 per cent, common acetic acid). To be used as the 

 last, washing out with ordinary water in order to obtain a blue- 

 violet tint of stain. The solution keeps better. 



290. Unna's Half-ripe Constant Stock Solution {Zeit. wiss. Mik., 

 viii, 1892, p. 483). 



Haematoxylin 



Alum . 



Alcohol 



Water 



Sublimed sulphur 



1 gm. 

 10 gm. 



100 c.c. 

 200 CO. 



2 gm. 



If the sulphur be added to the haematoxylin solution only when the 

 latter has become somewhat strongly blue, i.e. after two or three days' 

 time, the stage of oxidation attained by the solution will be fixed for 

 some time by the sulphur, and according to Unna the solution will 

 remain " constant " in staining power. Mayer (Mitth. Zool. Stat. 

 Neapel, xii, 1896, p. 309) finds that the sulphur process does not 

 preserve the solutions for long, whilst glycerin does. See below, 



" GlYCII/EMALUM." 



291. Mayer's Glychaemalum {Mitth. Zool. Stat. Neapel, xii, 1896, 

 p. 310). Haematein (or haemateate of ammonia), 0-4 grm. (to be rubbed 

 up in a few drops of glycerin) ; alum, 5 grm. ; glycerin, 30 ; distilled 

 water, 70. The stain is not purely nuclear, but may be made so by 

 washing out with alum solution or a weak acid. The solution keeps 

 admirably. 



Rawitz (Leitfaden, 2nd ed., p. 63) takes 1 grm. haematein, 6 grm. 

 ammonia alum, 200 grm. each of water and glycerin. 



Or (Zeit. Tviss. Mik., xxv, 1909, p. 391) 1 grm. haematein, 10 grm. 

 of nitrate of aluminium, 250 grm. each of water and glycerin. 



292. Hansen's Solution (Zool. Anz., 1895, p. 158). See fourth edition. 



293. Harris's Solution (Micr. Bull., xv, 1898, p. 47 ; Journ. App. 

 Mic., iii, p. 777). Alum-haematoxylin solution ripened by addition of 

 mercuric oxide. Mayer (GrundzUge, 1901, p. 171) finds the formula 

 " gives too much haematein." 



294. Bohmer's Haematoxylin {Arch. mik. Anat., iv, 1868, 

 p. 345 ; .lerzt. Intelligenzbl., Balern., 1865, p. 382). Make (a) a 

 solution of ha'matox. cryst. 1 gm., alcohol (absolute) 12 c.c, 

 and (b) alum 1 g.m., water 240 c.c. For staining, add two or 

 three drops of a to a watch-glassful of b. 



The alcoholic solution of haematoxylin ought to be old and dark 

 (§ 277). 



A. G. HoRNYOLD (Trans. Manch. Micr. Soc, 1915) prepares : — 

 Solution A : haematoxylin, 0-7 grm. ; absolute alcohol, 20 c.c. Solution 

 B : alum, 0-35 grm. ; aq. dest., 60 c.c. Mix A and B, expose to light 

 in window for three or four days, then add 20 drops of tincture of 

 iodine. Stain sections five to ten minutes till red-brown. Differentiate 

 in 70 per cent, alcohol, to which add a few drops of acetic acid. The 

 sections then turn blue. Good stain after osmic fixatives. 



