100 H .EM ATE IN 



298. Unna's Oxidised Haematoxylin (from Martinotti, Zeit. 

 wiss. Mik., xxvii, 1910. p. 31). Haematoxylin 0-5, alum 2, water 

 60, alcohol 10, glycerin 20, peroxide of hydrogen solution 10, 

 carbonate of soda 0-05. 



Martinotti, loc. cit., makes it u\) with hcvmatein (0-2 grm.). 



299. Apathy's Haematein Mixture I A {Mitth. Zool. Stat, 

 Neapel, xii, 1897, p. 712). Make (a) a solution of 9 per cent, 

 alum, 3 per cent, glacial acetic acid, and 0-1 per cent, salicylic 

 acid in water, and (b) a 1 per cent, solution of haematoxylin in 

 70 per cent, alcohol, preserved for from six to eight weeks in a 

 bottle not quite full. Mix 1 part of a with 1 of b and 1 of glycerin. 

 Stains either sections or material in bulk. Apathy uses it for 

 staining neuro-fibrils. 



300. Kleinenberg's Haematoxylin (Quart. Journ. Micr. Set., Ixxiv, 

 1879, p. 208). Highly irrational and very inconstant in its composition 

 and its effects ; see early editions ; also the criticism of IVIayer (Mitth. 

 Zool. Stat. Neapel, x, 1891, p. 174), and that of Squire in his Methods 

 and Formulce, p. 25, and the alternative formulae of Squire (loc. cit.) 

 and of VON Wistinghausen (Mitth. Zool. Stat. Neapel, x, 1891, p. 41). 



301. Mayer's Haemacalcium (Mitth. Zool. Stat. Neapel, x, 

 1891, p. 182). Haematein (or hasmateate of ammonia, §§ 278, 

 279), 1 grm. ; chloride of aluminium, 1 grm. ; chloride of calcium, 

 50 grm. ; glacial acetic acid, 10 c.c. (or common acetic acid, 

 20 c.c.) ; 70 per cent, alcohol, 600 c.c. Rub up finely together 

 the first two ingredients, add the acid and alcohol, dissolve either 

 cold or with heat ; lastly add the chloride of calcium. 



If the objects stain in too red a tone they may be treated with 

 a solution (of about 2 per cent.) of chloride of aluminium in 70 

 per cent, alcohol, or with a 0-5 to 1 per cent, solution of acetate 

 of soda or potash in absolute alcohol ; but washing with neutral 

 alcohol will generally suflice. 



With certain objects this solution does not penetrate well. 

 This may be remedied by acidifying the solution, or, which is 

 better, by leaving the objects for some time before staining in 

 acid alcohol. Anyway objects ought not to have an alkaline 

 reaction. If these precautions be taken, it will not be necessary 

 to use acid for washing out. 



The solution is not recommended as giving as good results as 

 haemalum, and Mayer recommends it merely as a substitute for 

 Kleinenberg's in cases in which an alcoholic haematein stain 

 seems indicated, as being easy to prepare, and constant in its 

 effects. 



302. Mayer's Haemastrontium (Grundzitge, Lee and Mayer, 

 1910, p. 106). One gramme haematein, 1 grm. aluminium chloride, 

 50 grm. strontium chloride, 600 c.c. alcohol of 70 per cent., and 

 (if desired) 0-25 grm. citric acid. Prepare and use as haemacalcium. 



