H MM ATE IN 161 



303. Dk Groot's Alcoholic Haemalum {Zeit. wiss. Mik., xxix, 1912, 

 p. 182). Mix 20 c.c. of glycerin with 24.0 of alcohol of 70 per cent. 

 Take 4 c.c. of the mixture, 2 c.c. of hydrogen peroxide, and 0-5 grm. of 

 haematoxylin, and dissolve with heat. Add 60 c.c. of the mixture, 

 4 grm. of calcium chloride, and 2 grm. of sodium bromide. Dissolve, 

 add 3 grm. of alum, heat and add 100 c.c. of the mixture. When the 

 alum is dissolved add 0-2 grm. of ferri-cyanide of potassium ; dissolve 

 and add 3 grm. more of alum and the rest of the mixture. Said to 

 stain almost as well as htemalum. Wash out with alcohol of 70 per 

 cent. 



304. Other Alumina-Haematein Solutions. A large number of 

 suppressed receipts will be found given in the earlier editions. 



305. R. HLeidenhain's Chrome Haematoxylin (Arch. mik. Anat., 

 xxiv, 1884, p. 468, and xxvii, 1886, p. 383). Stain for twelve to twenty- 

 four hours in a ^ per cent, solution of haematoxylin in distilled water. 

 Soak for the same time in a 0-5 per cent, solution of neutral chromate 

 of potash. W^ash out the excess of chromate with water. 



Objects that have been fixed in corrosive sublimate ought to be very 

 carefully washed out with iodine, or the like, as neutral hematoxylin 

 forms a black precipitate with any excess of sublimate that may remain 

 in the tissues. See Tornier, in Arch. mik. Anat., 1886, p. 181. 



The process is adajHed to staining in hulk. You can decolour the 

 objects to any extent by prolonging the soaking in the chromate. 

 Bichromate will do instead of the neutral chromate. 



306. Apathy's Modification of Heidenhain's Process {Zeit. wiss. 

 Mik., V, 1888, p. 47). This is an alcoholic method. Stain in a 1 per 

 cent, solution of ha;matoxylin in 70 or 80 per cent, alcohol. Differentiate 

 sections of 10 to 15 pi, half the time of staining, sections of 25 to 40 jjl 

 twice the time of staining, in 1 per cent, solution of bichromate of 

 potash in 70 to 80 per cent, alcohol, and wash out in alcohol of 70 per 

 cent. All these processes should be done in the dark. 



For celloidin series of sections, Apathy {ibid., 1889, p. 170) stains in 

 the haematoxylin solution as above for ten minutes ; then removes the 

 excess of haematoxylin fluid from the sections by means of blotting- 

 paper, and brings the series for from five to ten minutes into 70 per cent, 

 alcohol containing only a few drops of a strong (5 per cent.) solution of 

 bichromate. 



307. Schultze's Chrome Haematoxylin {Zeit. wiss. Mik., xxi, 1904, 

 p. 5). The tissues to be fixed for twelve or more hours in a bichromate 

 or chromic acid solution, preferably an osmium-bichromate mixture or 

 liquid of Flemming, then to be washed out for twenty-four hours in 

 50 per cent, alcohol in the dark and stained for twenty-four hours or 

 more in 0-5 per cent, haematoxylin in alcohol of 70 per cent., then 

 washed out in alcohol of 80 per cent. 



308. Hansen's Chrome Haematoxylin (ibid., xxii, 1905, p. 64). 

 Ten grm. of chrome alum boiled in 250 c.c. of water till green, and 

 1 grm. haematoxylin (dissolved in 15 c.c. of water) added ; to the 

 mixture when cold add 5 c.c. of sulphuric acid of 10 per cent, and (drop 

 by drop) a solution of 0-55 grm. of bichromate of potash in 20 c.c. of 

 water. Filter before use. Wash out with water free from air. 



309. Vanadium Haematoxylin (Heidenhain, Enzi/k. mik. Technik., 

 1903, p. 518). Add 00 c.c. of a 6 per cent, solution of haematoxylin 

 to a 0-25 per cent, solution of vanadate of ammonium (quantity not 

 stated ; should be 30 c.c, see Cohn in Ariat. Hefte, xv, 1895, p. 302). 

 The mixture to be used after three or four days ; it will not keep over 

 eight days. To be used with sections of sublimate material. A strong 

 plasma stain for special purposes, especially mucous glands. 



VADE-MECU.M. 6 



