162 HMMATEIN 



310. Benda's Copper Haematoxylin (Arch. mik. Anat., xxx, 1887, 

 p. 49), See fourth edition. According to our experience, not to be 

 compared with iron haematoxylin, and superfluous. 



311. Mallory's Phospho-molybdic Acid Haematoxylin {Anat. 

 Anz., 1891, p. 375). One part 10 per cent, phospho-molybdic 

 acid solution, 1 part haematoxylin, 100 parts water, and 6 to 10 

 parts chloral hydrate. Let the solution ripen for a week in sun- 

 light, and filter. Chiefly for central nervous system. Sections 

 should be stained for from ten minutes to one hour, and washed 

 out in two or three changes of 40 to 50 per cent, alcohol. It is 

 necessary that the solution should be saturated with haematoxylin 

 in order to obtain the best results ; if a good stain be not obtained 

 at once, more haematoxylin must be added. Water must never 

 be used for diluting it. 



See also Ribbert (Centralb. allg. Path., vii, 1896, p. 427 ; Zeit. wiss. 

 Mik., XV, 1898, p. 93), Patellani (Mon. Zool. Ital., xiii, 1902, p. 6), and 

 GoLOViN {Zeit. wiss. Mik., xix, 1902, p. 184). 



Sargent {Anat. Anz., xv, 1898, p. 214) quotes this stain, preceded by 

 mordanting for twenty-four hours in 5 per cent, sulphate of copper, as 

 Kenyon's. 



KoDis {Arch. mik. Anat., lix, 1901, p. 211) takes haematoxylin, 1 part ; 

 molybdic anhydride, 1-5 ; water, 100 ; U^O^, 0-5, or a crystal of HgO. 



Police {Arch. Zool. Napoli, iv, 1909, p. 300) takes 0-35 grm. haema- 

 toxylin, 10 drops phospho-molybdic acid of 10 per cent., 10 grm. chloral 

 hydrate, and 100 grm. alcohol of 70 per cent. 



312. Mallory's Phospho-tungstic Haematoxylin {Journ. Exp. 

 Med., V, 1900, p. 19 ; Zeit. wiss. Mik., xviii, 1901, p. 178) : 



Haematoxylin ...... 0-1 gm. 



Water 80-0 c.c. 



10 per cent, solution of (Merck's) phospho- 

 tungstic acid 20-0 c.c. 



Peroxide of hydrogen (U.S. Ph.) . . 0-2 c.c. 



(Dissolve the haematoxylin, add the acid, then the peroxide.) 

 Stain sections from two to twenty-four hours, wash out with water. 

 A pohjchromic stain, nuclei blue, intercellular substances pink. 

 We consider this a fine stain. 



313. Donaggio's Tin Haematoxylin {Ann. Nevrol. Napoli, xxii, 1904, 

 p. 192). A 1 per cent, solution of haematoxylin is poured slowly into 

 an equal volume of 20 per cent, solution of pink-salt (ammonia-chloride 

 of tin). Keep in the dark. 



314. Osmium Haematoxylin. Schultze {Zeit. zviss Mik., xxvii, 

 1910, p. 465) treats tissues for twenty-four hours or more with osmic 

 acid of 1 per cent., washes well with water, and puts for a couple of 

 days into ripened 0-5 per cent, solution of haematoxylin in alcohol of 35 

 to 50 per cent. Wash out for a day or more with alcohol of 70 per cent. 

 Intense plasma stain. 



315. .T. Anderson's Rapid Method of Ripening Haematoxylin 

 with Hypochlorite {Journ. Path, and Bad., 1923) : 



