HAMATE IN 163 



Stock Solutions 



A. Saturate boiling distilled water Avith ammonia (or potash) 



alum. Allow to cool and crystallise for twenty-four 

 hours. Filter. 



B. Shake up 2 grm. of commercial " chloride of lime " in 



100 c.c. of distilled water. Allow to stand for four 



hours with occasional shaking. Filter. Or use a 2 per 



cent, solution of chloramine T. 



Shake up 0-25 grm. of ha^matoxylin in 5 c.c. of absolute alcohol, 



add 20 c.c. solution B. Mix for a few seconds. Add this dark 



brown solution to 70 c.c. of solution A with constant shaking. 



Add 5 c.c. glacial acetic acid. 



The haematoxylin is then ready for use. If it becomes purplish 

 or bluish add more acetic acid. The stain that has been used 

 may be filtered back into the bottle. It is not usually necessary 

 to filter the stain on to the slide. This haematoxylin stains very 

 rapidly ; two to three minutes is usually ample. 



316. H. E. Shortt's Rapid Method for Heidenhain Stain {Ind. 

 Jour. Med. R., 1923). The procedure is as follows : — - 



To 95 c.c. of distilled water in a flask one adds 1 grm. of pure 

 haematoxylin crystals (Griibler for preference). This solution is 

 slowly brought to the boiling-point, with occasional shaking to 

 complete solution of the haematoxylin, and at this stage 5 c.c. of 

 pure carbolic acid, liquefied if necessary, are added. 



The solution is now allowed to cool and is then ready for use. 

 Extensive trial, under varied conditions of temperature and 

 climate, of solutions so prepared has shown that this stain is 

 absolutely reliable in use, and the addition of the carbolic acid 

 seems to increase the power of penetration so that by following 

 the usual technique an intense nuclear stain is obtained with 

 unfailing regularity. 



317. Held's Molybdic Acid Haematoxylin, see § 815. 



6—2 



