166 PLASMA STAINS 



In recent years this stain has been much used as a counterstain 

 after iron alum hasmatoxyhn. Saturate absokite alcohol and 

 pass the slides through in their way to xj'lol or benzol balsam. 



Saurefuchsin and Orange G. Lee has had good results by 

 mixing the aqueous solutions of these two dyes, but unfortunately 

 has not noted the proportions. Squire {Methods and Formulce, 

 p. 42) takes 1 grm. Saurefuchsin, 6 grm. Orange G in 60 c.c. of 

 alcohol and 240 c.c. of water. See also under " Connective 

 tissues." 



322. Ehrlich-Biondi Mixture (or Ehrlich-Biondi- 

 Heidenhain Mixture) {Pfluger's Arch., xliii, 1888, p. 40). 



To 100 c.c. saturated aqueous solution of orange add with 

 continual agitation 20 c.c. saturated aqueous solution of Saure- 

 fuchsin {Acid Fuchsin) and 50 c.c. of a like solution of methyl 

 green. 



(According to Krause {Arch. mik. Anat., xlii, 1893, p. 59), 100 parts of 

 water will dissolve about 20 of Saurefuchsin (Rubin S), 8 of orange G 

 and 8 of methyl green.) The solutions must be absolutely saturated^ 

 which only happens after several days. 



Dilute the mixture with 60 to 100 volumes of water. The 

 dilute solution ought to redden if acetic acid be added to it ; and 

 if a drop be placed on blotting-paper it should form a spot bluish- 

 green in the centre, orange at the periphery. If the orange zone 

 is surrounded by a broader red zone, the mixture contains too 

 much fuchsin. 



According to M. Heidenhain (" Ueber Kern u. Protoplasma," 

 in Festchr.f. KolUker, 1892, p. 115) the orange to be used should 

 be " Orange G," the Acid Fuchsin or Saurefuchsin should be 

 " Rubin S " (" Rubin " is a synonym of Fuchsin) and the methyl 

 green should be " Methylgriin 00." And Lee thought it absolutely 

 necessary that these ingredients be those prepared under those 

 names by the Actienfabrik fiir Anilinfabrikation in Berlin. 



The strong solutions- directed to be taken readily precipitate 

 on being mixed. To avoid this it is recommended by Squire 

 {Methods and Formulce, etc., p. 37) to dilute them before mixing. 



Other proportions for the mixture have been recommended by 

 Krause {loc. cit. supra), viz. 4 c.c. of the Saurefuchsin solution, 7 of the 

 orange G and 8 of the methyl green ; the mixture to be diluted 50 to 

 100-fold with water. Thome {Arch. mik. Anat., lii, 1898, p. 820) gives 

 the proportions 2:5:8, and dilutes 100-fold. 



Stain sections (N.B. sections only) for six to twenty-four hours. 

 Dehydrate with alcohol, clear with xylol, and mount in xylol 

 balsam. 



In the intention of the observers who have elaborated this 

 stain it is a jjrogressive stain, and not a regressive one. It does 

 not require any differentiation, and the sections should be got 



