172 PLASMA STAINS 



XV, 3, 1899, p. 338) it may be used for intra-vitam staining of 

 tissues in the same way as methylen blue, by injection or immersion 

 with contact of air. It is especially a granule stain. Similar 

 results are recorded by Arnold (Anat. Anz., xvi, 1899, p. 568, 

 and xxi, 1902, p. 418). See also Ehrlich and Lazarus, Ancemie, 

 i, 1898, p, 85 ; Loisel (Journ. de VAnat. et de la Physiol., 1898, 

 pp. 197, 210, 217) {intra-vitam staining of sponges) ; and 

 Prowazek {Zeit. iviss. ZooL, Ixii, 1897, p. 187) {intra-vitam 

 staining of Protozoa), We have had very good results with it as 

 an ifitra-vitani stain. 



It has been found useful for staining, in hardened material, 

 the corpuscles of Nissl {q.v.) in nerve-cells, S, Mayer {Lotos, 

 Prague, 1896, No. 2) states that it also stains degenerating myelin. 

 The solutions that have been employed for staining fixed material 

 are strong aqueous ones, 1 per cent, to concentrated. (See also 

 §§ 766 et seq.). 



336. Biebrich Scarlet (British Dyes, Ltd., Huddersfield). 

 A. K. Gordon {British Medical Journ., 1917, p. 828) finds this 

 an excellent acid dye which never overstains and is not diffuse. 

 Use in 1 per cent, solution, or as recommended by Scott. 



We have used this stain a good deal and find it better than 

 any other plasma dye that we have tried, 



337. The Eosins, found in commerce under the names of Eosin, 

 Saffrosin, Primerose Soluble, Phloxin, Bengal Rose, Erythrosin, 

 Pyrosin B, Rose B, a I'Eau, etc., are all " acid " phthalein colours. 

 They are not quite identical in their properties. Most of them 

 are soluble both in alcohol and in water, but some only in alcohol 

 (" Primerose a VAlcool "), 



According to Conn {op. cit.) the proper dye is ethyl eosin, and when 

 ordering supplies, this name, and not alcohol soluble eosin, should be 

 used. Solubility at 26° C, in water 03 per cent., in alcohol 113 per 

 cent. 



They are all diffuse stains, formerly much used as contrast 

 stains, less so now, Hansen {Anat. Hefte, xxvii, 1905, p, 620) 

 adds 1 drop of acetic acid of 2 per cent, to 9 c,c, of 1 per cent, 

 eosin, which makes the stain more selective. 



Eosin is a stain for red blood-corpuscles, and also for certain 

 granules of leucocytes (see under " Blood "). 



The yolk of some ova takes the stain strongly, so that it is 

 useful in some embryological researches. 



338. For Bengal Rose see Griesbach, Zool. Anz., 1883, p, 172, 



339. EnRLicii's Indulin-Aurantia-Eosin, or Acidophilous 

 Mixture, or Mixture C, or Mixture for Eosinophilous Cells (from 

 the formula kindly sent Lee by Dr. Grubler), Indulin, aurantia, 

 and eosin, of each 2 parts ; glycerin, 30 parts. This gives a very 

 quick, syrupy solution. To use it, coverglass preparations may 



