PLASMA STAINS 173 



be floated on to it ; or sections on slides may have a few drops 

 poured on to them, the sHde being laid flat till the stain has taken 

 effect (twenty-four hours for Flemming material). Lee found 

 that with Flenuuing material it gives a powerful and good stain, 

 which is much more resistant to alcohol than that of the Eiirlich- 

 BiONDi mixture, and is, therefore, much more adapted to ordinary 

 work. The stain keeps well. 



Israel {Prakiik. Path. Hist., Berlin, 1893, p. 68) gives a more com- 

 plicated receipt. 



340. Methyl Green and Eosin (Calberla, Morph. Jahrh., iii, 1877, 

 Heft o, p. 625 ; List, Zeit. iviss. Mik., ii, 1885, p. 147 ; Balbiani, Ann. 

 Microgr., Paris, vii, 1895, p. 245 ; Rhumbler, Zeit. wiss. ZooL, Ixi, 1895, 

 p. 38). See early editions. 



341. Methylen Blue and Eosin (Chenzinsky, quoted from Zeit. wiss. 

 Mik., xi, 2, 1894, p. 269). 



Metliylen blue, sol. sat. in water . . .40 c.c. 



Eosin, 0-5 per cent, in 70 per cent, alcohol . 20 c.c. 



Distilled water, or glycerin . . . . 40 c.c. 



This solution will only keep for about eight days. 



PiANESE (ibid., xi, 1894, p. 345) adds a considerable proportion of 

 carbonate of lithium. 



See also the mixture of Bremer (Arch, mik, Anat., xlv, 1895, p. 446). 

 Lee has tried Chenzinsky's mixture as a tissue stain, without good 

 results ; but see Rosin, Berliner klin. WocJienschr., 1898, p. 251 ; Zeit. 

 zviss. Mik., xvi, 1899, p. 223, and xvii, 1900, p. 333. 



See also Laurent (Centralb. allg. Path., xi, 1900, p. 86 ; Zeit. wiss. 

 Mik., xvii, 1900, p. 201). 



342. Mallory's Eosin and Methylen Blue (Journ. Med. Research, 

 January, 1904). Sections of Zenker material (other sublimate material 

 not so good) are stained for half to three-quarters of an hour at 56° C. 

 in 5 per cent, aqueous solution of eosin, rinsed and flooded with solution 

 of 1 part of methylen blue, and 1 of potassium carbonate in 100 of 

 water, diluted with about 7 parts of water. After forty minutes they 

 are flooded (not washed) with water, and differentiated for about five 

 minutes in alcohol of 95 per cent. Absolute alcohol, xylol, balsam. 



343. Other Eosin and Methylen-blue Stains. For some very 

 im])ortant ones see under " Blood." 



344. Light Green (Lichtgrun S. P.). An " acid " colour, 

 soluble in alcohol, and a good plasma stain. 



Solubility at 26° C, in water 20-35 per cent., in alcohol 0-82 per cent. 



Benda {Verh. physiol. Ges. Berlin, December 18th, 1891, Nos. 

 4 u. 5) stains sections for twenty-four hours in anilin-water 

 safranin solution, then for about half a minute in a solution of 

 0*5 grm. Lichtgrun or Saureviolctt (Griibler) in 200 c.c. of alcohol 

 dehydrates and mounts in balsam. This ])roeess gives a very 

 elegant stain, but requires very thin sections, and there is always 

 risk of the safranin being washed out. The Lichtgrim stain 

 unfortunately does not keep at all well. 



See also Prenant, Arch. mik. Anat., vii, 1905, p. 4,'30, and 



