174 PLASMA STAINS 



GuiEYSSE, C.R. Soc. Biol., Ixii, 1907, p. 1212, and Bailey {Jour. 

 Med. Res., 1920). 



345. Janus Green (Michaelis, Arch. tnik. Anat., Iv, 1900, 

 p. 565). Used in solution of 1 : 30,000 for staining mitochondria 

 (pancreas, salivary glands, etc.) in the fresh state. For method 

 of using refer to § 761, p. 339. 



Solubility at 26° C, in water 518 per cent., in alcohol 112 per cent. 



346. Malachite Green (syn. Solid Green, Victoria Green, New Green, 

 Benzoyl Green, Fast Green). A basic colour, which has been used as a 

 plasma stain for the ova of Ascaris by van Beneden and Neyt. These 

 authors used it for glycerin preparations ; it can hardly be got into 

 balsam. 



Flemming {Arch. mik. Ayuit., xix, 1881, p. 324) attributes to it a 

 special affinity for nucleoli. 



347. Iodine Green (" Hofmann's Griin "), see Griesbacu {Zool. 

 Anz., No. 117, vol. v, 1882, p. 406). Stain essentially that of methyl 

 green, but plasma often violet through the presence of a violet impurity 

 (Mayer, Mitth. Zool. Stat. Neapel, xii, 1896, p. 311 ; see also earlier 

 editions). It is now only used by botanists. 



348. Thiophen Green (Thiophengrun), see KnwsE, Intern. Monatsschr. 

 Anat., etc., iv, 1887, Heft. 2. 



349. Coerulein S., a green " acid " dye, is recommended for the 

 staining of muscle-fibrils by M. v. Lenhossek {Anat. Anz., xvi, 1899, 

 p. 339). See also Heidenhain, ibid., xx, 1901, p. 37, and Rawitz, ibid., 

 xxi, 1902, p. 554. 



350. Quinolein Blue (Cyanin, Chinolinblau ; v. Ranvier, Traite, 

 p. 102). Quinolein is said by Ranvier to have the property of staining 

 fatty matters an intense blue. 



It is useful for staining Infusoria, which in dilute solution it stains 

 during life. See the methods of Certes. 



From the reactions mentioned by Ranvier it would seem that his 

 " bleu de quinoleine " is not the preparation that usually goes under 

 that name. See Ehrlich, in Arch. tnik. Anat., xiii, 1877, p. 266. 



351. Indulin and Nigrosin. Indulin, Nigrosin, Indigen, Coupler's 

 Blue, Fast Blue R, Fast Blue B, Blackley Blue, Guernsey Blue, Indigo 

 substitute are the names of brands of a group of dyes, mostly " acid," 

 related to the base violanilin. According to Behrens the name Indulin 

 is generally given to a bluish brand, and that of Nigrosin to a blacker 

 one. 



Nigrosin, used with sublimate material, Lee found stains both nuclei 

 and cytoplasm, the chromatin strongly. It will not give the stain at all 

 with chrome-osmium material. 



According to Calberla {Morph. Jahrb., iii, 1877, p. 627) the concen- 

 trated aqueous solution of Indulin should be diluted with 6 volumes 

 of water. Sections will stain in the dilute solution in from five to 

 twenty minutes. He also says that it never stains nuclei ; the 

 remaining cell-contents and intercellular substance are stained blue. 

 This seems to us to be, roughly, correct. 



352. Safranin and Nigrosin (or Indigo-Carmine) (Kossinski, Zeit. 

 wiss. Mik., vi, 1880, p. 61). See early editions. 



353. Picro-Nigrosin, Pfitzer {Deutsch. Botan. Gesellsch., 1883, 

 p. 44) dissolves nigrosin in a saturated solution of picric acid in 

 water, and uses it for fixing and staining at the same time, on the 

 slide. See also under " Connective Tissues." 



