PLASMA STAINS 175 



354. Anilin Blue. Under this title are comprised various 

 " basic " derivatives of the base rosaniliu. They occur under 

 the names Spirit Soluble Blue (Bleu Alcool), Gentian Blue 6 B, 

 Spirit Blue 0, Opal Blue, Bleu de Nuit, Blue Lumiere, Parma 

 Bleu, Bleu de Lyon. Some authors give tlie name Bleu de Nuit 

 and Griindstiehblaii as synonyms of Bleu de Lyon. The Encycl. 

 mik. Technik. says it is " AniHnblau B — 6 B," with many synonyms 

 or designations of brands, Parma blue being " Anilinblau R or 

 2 R." 



Conn (op. cit.) writes : " Anilin blue W.S. should be regarded as a 

 group of dyes rather than a simple dye. The composition of the various 

 commercial products sold under this name is uncertain." Conn mentions 

 that " Water Blue " (see next paragraph) is also a synonym for Anilin 

 Blue. The name " Cotton Blue " is also applied to '• Methyl Blue," 

 and " Anilin Blue." 



Lee found it a fairly good stain, giving very good differentiations 

 of nerve-tissue and of cartilage (as has already been pointed out by 

 Baumgarten and by Jacoby). Maurice and Schulgin stain 

 in bulk with it after borax-carmine, using a very dilute alcoholic 

 solution. Baumgarten and Jacoby stain sections in a 0-2 per 

 cent, alcoholic solution. 



ToNKOFF {Arch. mik. Anat., Ivi, 1900, p. 394) adds a little 

 tincture of iodine to the solution of the dye, or mordants the 

 sections with iodine. 



Skrobaxsky {Intern. Monatsschr. Anat., xxi, 1904, p. 20) uses 

 it in water with picric acid. 



355. Carmine Blue (Bleu Carmin Aqueux, from Mesiter, Lucius, and 

 Brunig, at Hochst-a-M.). Janssens {La Cellule, ix, 1893, p. 9) states 

 that this colour possesses a special affinity for the parts of cytoplasm 

 that are undergoing cuticular differentiation. He uses it in alcoholic 

 solution acidified. 



356. Methyl Blue. Under this title are comprised some other 

 derivatives of the base rosanilin. They are " acid " colours. 

 Here belong Methyl Blue, Cotton Blue, Water Blue (Wasserblau) 

 Methyl Water-Blue, China Blue (Chinablau), Soluble Blue. See 



§ above. 



Amongst these Water Blue (Wasserblau) possesses some useful 

 properties. According to Mitrophanow (quoted from Zeit. wiss. 

 Mik., V, 1888, p. 513), used in concentrated aqueous solution it 

 gives a very good double stain with safranin. It is very resistant 

 to alcohol. Using the Wasserblau hrst, and then the safranin, 

 Lee has had some interesting results. The Wasserblau must be 

 used first. With chrome-osmium material, twelve to twenty- 

 four hours in the blue, and four or five in the safranin may not 

 be too much. Lee's stains have not kept well. 



Manx {Methods, etc., p. 216) uses a mixture of 35 parts 1 per 

 cent, solution of eosin, 45 of methyl blue 1 per cent., and 100 of 



