PLASMA STAINS 177 



A double stain by means of artificial Alizarin, or Alizarin-cyanin, 

 requiring the use of special mordants supplied by the colour manu- 

 facturers, and very complicated. Hee fifth edition. 



Rawitz {Zeit. wiss Mik., 1909, pp. 393 and 395) also recommends 

 a solution of 1 grm, of Saure-Allzarinblau BB (or Sauregriin G) (both 

 from Hochst), 10 grm. ammonia alum, 100 c.c. glycerin, and 100 c.c. 

 water. 



SziJTZ {ibid., xxix, 1912, p. 289) fixes in a mixture of 15 c.c. 1 per 

 cent, platinum chloride, 15 c.c. formol, and 30 c.c. saturated solution of 

 sublimate, makes paraffin sections, and stains them with Heidenhain's 

 iron-haematoxylin. They are then treated for from five to six hours 

 with 5 per cent, solution of aluminium acetate, rinsed, and stained for 

 from five to six hours with Benda's sulphalizarinate of soda (given 

 under " Mitochondria "), and got into balsam. A red plasma stain, 

 affecting plasmafibrils. For intra-vitarn stains with alizarin see 



FiSCHEL, NiLSSON, Zool. AuZ., XXXV, 1909, p. 196. 



361. For Benda's Alizarin Stains, see under " Centrosomes," 

 " Mitochondria," and " Neuroglia," §§ 697, 1085. 



