188 NUCLEAR STAINS, COAL TAR 



With Toluidin Blue Lee has had some superb stains of chromatin, 

 unfortunately accompanied by a diffuse staining of cytoplasm. 



Mann {Zeit. zviss. Mik., xi, 1894, p, 489) states that he has had good 

 results by staining with it after eosin. 



See further, as to the micro-chemical properties of this dye, Harris, 

 The Philadelphia Medical Journal, May 14th, 1898. It much resembles 

 methylen blue. 



Metzner (Nagel's Handb. Phys., ii, 1907, p. 915) mordants sections 

 before staining, for three-quarters of an hour in iron almn. 



Magdala Red (Naphthalin Red, Rose de Naphthaline). 



Fuchsin (meaning the basic fuchsins, a series of Rosanilin salts 

 having very similar reactions, and found in commerce under the names 

 of Fuchsin, Anilin Rkd, Rubin, Rosein, Magenta, Solferino, 

 Corallin). Graser {Deutsche Zeit. Chirurgie, xxvii, 1888, pp. 538 — 

 584 ; Zeit. zviss. Mik., v, 1888, p. 378) stains for twelve to twenty-four 

 hours in a dilute aqueous solution, washes out for a short time in alcohol, 

 stains for a few minutes in aqueous solution of methylen blue, and 

 dehydrates with alcohol. A double stain. Chromatin and nucleoli red, 

 all the rest blue. 



Ziehl's Carbolic Fuchsin {Zeit. zviss. Mik., vii, 1890, p. 39) 

 consists of basic fuchsin 1 grm., acid, carbol. crist. 5 grm., alcohol 

 10 grm., aq. dest. 100 grm. The stain is differentiated with 

 alcohol followed by clove oil. 



Kresofuchsin (Rothig, Arch. mik. Anat., Ivi, 1900, p. 354). Its 

 aqueous solution is red and stains mucus, cartilage, keratin, and nuclei 

 red, whilst its alcoholic solution is blue and stains elastin blue. See 

 also under " Connective tissues." 



Bismarck Brown has this advantage, that being sufficiently resistant 

 to alcohol it may be utilised for staining entire objects. 



Kaiser {Biblioth. Zool., H. 7, 1 Halft, 1891 ; Zeit. zviss. Mik., viii, 

 1891, p. 363) stains for forty-eight hours, and at a temperature of 60° C. 

 in saturated solution of Bismarck brown in 60 per cent, alcohol (the 

 solution to be made in boiling alcohol), and washes out (until all is 

 decoloured except the karyokinetic figures) in 60 per cent, alcohol, 

 containing 2 per cent, hydrochloric acid or 3 per cent, acetic acid. 



Methyl Violet. See ante, § 373. 



Benzoazurin (Martin, Zeit. zviss. Mik., vi, 3, 1889, p. 193). Stain for 

 an hour or so in dilute aqueous solution, and wash out with HCI alcohol. 



Methylen Blue. 



Nigrosin (Errera, Proc.-Verb. Soc. Beige de Mik., 1881, p. 134) gives 

 a good stain which resists alcohol well. 



Methyl Green is sometimes useful in certain mixtures (see next 

 chapter). 



