METHYLEN BLUE 199 



to keep the required alcoholic solution of fuchsiu. and to add the 

 phenol and anilin oil when it is desired to make up the stain anew. 

 If sections are stained in the solution for an hour or more they 

 gradually become useless. In our experience it is safe to stain the 

 sections as long as half an hour, but the best results are obtained 

 in ten minutes. 



" Wash off the excess stain in running water, blot with filter 

 paper, and decolorise in 95 per cent, alcohol until the sections 

 become pink. Wash off in water and counterstain 15 to 60 

 seconds with Loeffler's methylen blue. Wash in water. Dehy- 

 drate and decolorise for a few seconds in absolute alcohol (until 

 the excess blue is removed). Clear in xylol ; mount in balsam." 



