214 OTHER STAINS 



2 grni. of glacial acetic acid, and 100 c.c. of distilled water, washes 

 in distilled water, and passes rapidly through absolute alcohol to 

 thick cedar oil, in which the preparations remain definitely 

 mounted. Nuclei blue, protoplasm red. 

 Its principal use is for Unna's elastin stain. 



See also " Connective Tissues " in Part II, and Laurent, Zeit. wiss. 

 Mik., xiii, 1896, p. 302 ; Ruzicka, ibid., xiv, 1898, p. 455 ; and Wolff, 

 ibid., xix, 1903, p. 488. 



420. Purpurin, see Ranvier's Trciite technique, p. 280 ; Duval's 

 Precis de Teclinique histologique, p. 221 ; and Grenacher's formula in 

 Arch. mik. Anat., xvi, 1879, p. 470. A very weak stain. 



421. Indigo. Indigo is employed in histology in the form of solutions 

 of so-called indigo carmine, or sulphindigotate of soda or potash. The 

 simple aqueous solution gives a diffuse stain, but is of use when employed 

 in conjunction with carmine. See below. 



Thiersch's Oxahc Acid Indigo-Carmine (see Arch. mik. Anat., i, 1865, 

 p. 150). 



422. Other Vegetal Dyes. See early editions. Those recommended 

 by Claudius {Zeit. wiss. Mik., xvii, 1900, p. 52) are superfluous. 



CARMINE COMBINATIONS 



423. Seiler's Carmine followed by Indigo-Carmine {Am. Quart. Mic. 

 Journ., i, 1879, p. 220). Stain in borax-carmine, wash out with HCl 

 alcohol, wash out the acid, and after-stain in an extremely dilute alcoholic 

 solution of indigo-carmine (2 drops of saturated aqueous solution added 

 to an ounce of alcohol and filtered). 



Lee found this method gave good results with sections, but not if it 

 was attempted to stain in bulk. 



424. Merkel's Carmine and Indigo-Carmine in One Stain (Merkel, 

 Unters. anat. Anst. Rostock, 1874 ; Month. Mic. Journ., 1877, pp. 242 

 and 317). 



Also Norris and Shakespeare, Amer. Journ. Med. Sci., January, 

 1877 ; Merkel, Mon. Mic. Journ., 1877, p. 242 ; Marsh, Section 

 Cutting, p. 85 ; Bayerl, Arch. Mik. Anat., xxiii, 1885, pp. 36, 37 ; 

 Macallum, Trans. Canad. Instit., ii, 1892, p. 222 ; Journ. Roy. Mic. 

 Soc, V, 1892, p. 698. 



425. Mayer's Carmalum (or Haemalum) and Indigo-Carmine in 

 One Stain. Mayer {Mitth. Zool. Stat. Neapel, xii, 1896, p. 320) 

 obtains very good results by taking a solution of 0-1 grm. of 

 indigo-carmine in 50 c.c. of distilled water, or 5 per cent, alum 

 solution, and combining it with from 4 to 20 volumes of carmalum 

 or haemaluin. 



426. Carmine and Picro-Indigo-Carmine (Ramon y Catal, 

 Rev. de Sienc. med., 1895 ; Calleja, Rev. trim. Microgr., ii, 

 1897, p. 101 ; Zeit. wiss. Mik., xv, 1899, p. 323). For use after 

 a carmine stain, Ramon takes a solution of 0-25 grm. of indigo- 

 carmine in 100 grm. saturated aqueous solution of picric acid. 

 Stain {sections) for five to ten minutes, wash in weak acetic acid, 

 then in water, then remove the excess of picric acid with absolute 

 alcohol, clear and mount. 



