OTHER STAINS 215 



Ramon also {FAementos de Ilistnlogia. 1897 ; quoted from La 

 Cellule, xix, 1901. p. 212) employs the piero-iiidi<fo mixture after 

 Magenta ; stain strongly in saturated solution of magenta, rinse 

 in water until no more colour comes away, and pass into the 

 indigo mixture. See also Borrel, Ann. Inst. Pasteur, 1901, 

 p. 57, or Lee et Henneguy, Traite, p. 268. 



427. Carmine and Anilin Blue (or Bleu Lumiere, or Bleu de Lyon) 

 (Duval, Precis de Technique Microscopique, 1H78, p. 225). Stain 

 with carmine ; dehydrate, and stain for a few minutes (ten 

 minutes for a section of nerve-centres) in a solution of 10 drops 

 of saturated solution of anilin blue in alcohol to 10 grm. of absolute 

 alcohol. Clear with turpentine, without further treatment with 

 alcohol, and mount in balsam. 



Other authors recommend, instead of anilin blue, bleu de 

 Lyon, dissolved in 70 per cent, alcohol acidulated with acetic 

 acid (Maurice and Sciiulgin), or bleu lumiere. 



The solutions of both these colours should be extremely dilute 

 for sublimate material, but strong for chrome-osmium material. 

 It is possible to use them for staining in bulk. 



Baumgarten {Arch. mik. Anat., xl, 1892, p. 512) stains sections 

 (of material previously stained in borax-carmine) for twelve 

 hours in a 0-2 per cent, solution of bleu de Lyon in absolute 

 alcohol, and washes out for about half that time before mounting 

 in balsam. He recommends the process for cartilage and nerve- 

 centres. 



428. Carmine and Malachite Green. Maas (Zeit. wiss. ZooL, 1, 4, 

 1890, p. 527) recommends borax-carmine followed by weak alcoholic 

 solution of malachite green, with a final washing out with stronger 

 alcohol. 



429. Carmine and Picro-nigrosin (Pianese). See Journ. Boy. Mic. 

 Soc, 1892, p. 292. 



430. Carmine and Picric Acid. See § 266. 



H^MATEIN OR HyEMATOXYLIN COMBINATIONS 



431. Haematoxylin and Picric Acid. See § 325. 



432. Haematoxylin and Eosin. This popular combination gives 

 results that are aesthetically beautiful, but (for most objects) is 

 not so useful as many others, the eosin lacking in electivity. 

 Objects may be stained with haematoxylin (either in the mass or 

 as sections) and the sections stained for a few minutes in eosin. 

 Lee thinks it is better to take the eosin weak, though it has been 

 recommended (Stohr, see Zeit. wiss. Mik., i, 1884, p. 583) to 

 take it saturated. Either aqueous or alcoholic solutions of eosin 

 may be used. 



HiCKSON {Quart. Journ. Mic. Sci., 1893, }). 129) stains sections 

 for one hour in a strong solution of eosin in 90 per cent, alcohol, 



