216 OTHER STAINS 



washes with alcohol, and stains for twenty minutes in a weak 

 solution of haeniatoxylin. 



This method is most particularly recommendable for embryo- 

 logical sections, as vitellus takes the eosin stain energetically, 

 and so stands out boldly from the other germinal layers in which 

 the blue of the haeniatoxylin dominates. 



See also List {Zeit. iviss. Mik., ii, 1885, p, 148) ; Buscii (Verh. 

 Berl. Phys. Ges., 1887) ; Gierke {Zeit. wiss. Mik., i, 1884, p. 505). 



Sections should be very well washed before being passed from 

 eosin into hasmatoxylin or the reverse, as eosin very easily pre- 

 cipitates hfematoxylin. 



For the complicated and superfluous mixtures of Renaut and 

 of EvERARD, Demoor and Massart, see Foi.'s Lehrbuch, p. 196, 

 Ann. Inst. Pasteur, vii, 1893, p. 166, or early editions. 



433. Haematoxylin and Congo. See § 332. 



434. Haematoxylin and Safranin. Rabl {Morph. Jahrb., x, 1884, 

 p. 215) stained very lightly with very dilute Delafield's haematoxylin for 

 twenty-four hours, then for some hours in (Pfitzner's) safranin, and 

 washed out with pure alcohol. The plasma stain is here given by the 

 haematoxylin. 



Similarly Regaud, Verh. Anat. Ges., xiv, 1900, p. 112, 

 FoA {Festschr. Virchow, 1891, p. 481) stains in a mixture of 

 25 c.c. of Boluiier's haematoxylin, 20 of 1 per cent, solution of 

 safranin, and 100 of water for one to three minutes. 



435. Haematoxylin and Saurefuchsin. Stain first with iron 

 hcTcmatoxylin or hasmalum, then stain (sections) in 0-5 per cent, 

 aqueous solution of Saurefuchsin, dehydrate and mount. 



436. Haematoxylin and Saurefuchsin and Orange. Proceed as 

 above, using for the second stain the following mixture : Saure- 

 fuchsin, 1 grm. ; orange, 6 grm. ; rectified spirit, 60 c.c. ; water, 

 240 c.c. (from Squire's Methods and Formuke, p. 42). Using 

 orange G (not mentioned by Squire), we have had very good results. 



The method of Cavazzani {Riforma Med., Napoli, 1893, p. 604 ; 

 Zeit. zviss. Mik., xi, 3, 1894, p. 344) is far too complicated. 



437. Haematoxylin and Picro-Saurefuchsin (van Gieson, New 

 York Med. Journ., 1889, p. 57 ; quoted from Moeller, Zeit. 

 zviss. Mik., XV, 2, 1898, p. 172, which see for further details). 

 Proceed as above, using for the second stain the picro-Saure- 

 fuchsin mixture, § 326. The second stain must not be too pro- 

 longed. 



Weigert (Zeit. wiss. Mik., xxi, 1904. p. 1) stains first in his 

 iron-hannatoxylin mixture (§ 284), rinses in water, and stains 

 for a short time in his picro-Saurefuchsin (§ 326), rinses, dehy- 

 drates with 90 per cent, alcohol, and clears with carbolic acid- 

 xylol mixture (1 : 3). 



