234 INJECTION 



Bayliss (in personal communication) suggests for prevention 

 of coagulation, to wash out in citrate of soda (4 per cent.) instead 

 of 0-75 NaCl, or to add \ per cent, oxalate of calcium to 0-75 per 

 cent. NaCl, To relax arterial walls, add sodium nitrite 1 in 500 

 to the washing out fluid. 



Or, morphia may be added to the injection mass, or 1 per cent, 

 of lactic acid. Mozejko {Zeit. wiss. Mik., xvi, 1909, p. 545) 

 prefers a saturated solution of neutral Peptonum siccum, which 

 has the advantage of hindering coagulation. For warm-blooded 

 animals the mass should be warmed to body-temperature ; and 

 in all cases masses that tend to dehydrate tissues should be avoided 

 if possible. 



ROBIN'S MASSES 



517. Robin's Gelatin Vehicle {Traite, p. 30). One part of 

 gelatin soaked and melted in 7, 8, 9, or even 10 parts of water, 

 on a water-bath. 



This vehicle, like all gelatin masses, is liable to be attacked by 

 mould if kept long ; camphor and carbolic acid do not suffice to 

 preserve it. Chloral hydrate 2 per cent, is said to do so. 



518. Robin's Glycerin-Gelatin Vehicle (Traite, p. 32). Dissolve 

 in a water-bath 50 grm. of gelatin in 300 grm. of water, in which 

 has been dissolved some arsenious acid ; add of glycerin 150 

 grm., and of carbolic acid a few drops. Unlike the pure gelatin 

 vehicles, this mass does keep indefinitely. 



Frankl (Zeit. f. wiss. Zool., Ixiii, 1897, p. 28) prepares a similar 

 vehicle, and adds to it a little solution of corrosive sublimate and a 

 crystal of thymol. 



519. Robin's Carmine Colouring Mass (Traite, p. 33). Rub up 

 3 grm. of carmine with a little water and enough ammonia to 

 dissolve it. Add 50 grm. of glycerin and filter. 



Take 50 c.c. of glycerin with 5 c.c. of acetic acid, and add it 

 by degrees to the carmine-glycerin, until a slightly acid reaction 

 is obtained (as tested by very sensitive blue test-paper, moistened 

 and held over the mixture). 



One part of this mixture is to be added to 3 or 4 parts of the 

 vehicles given above. 



520. Robin's Ferrocyanide of Copper Colouring Mass (ibid., p. 34). 

 Take— 



(1) Ferrocyanide of potassium (concentrated 



solution) . . . . .20 c.c. 



Glycerin . . . . . . 50 ,, 



(2) Sulphate of copper (concentrated solu- 



tion) . . . . . . 35 „ 



Glycerin . . . . . . 50 ,, 



Mix (1) and (2) slowly, with agitation ; at the moment of injecting 

 combine with 3 volumes of vehicle. 



