732 



APPENDIX II 



Since this solution should also be freshly made just before use, 

 it is convenient to keep the following solutions always made up. 



NaCl . 

 KCl . 

 CaClg . 

 NaHCO. 



9% 



1% 



1% 

 10% 



Immediately before use 20 c.c. NaCl, 4 c.c. KCl, 4 c.c. CaCl2 are 

 added to 200 c.c. aqua dest. If sterility is desired, the above 

 solution is heated to boiling. After cooling, 0-4 c.c. NaHCOg is 

 added. 



N.B. A solution containing NaHCOg must on no account be 

 heated, since by loss of COg the bicarbonate becomes transformed 

 into the carbonate. If desired, it may be filtered through a 

 Berkefeld filter. 



Tyrode 



NaCl . 

 KCl . 

 CaCl2 . 

 MgCfa . 

 NaH2P04 

 NaHCO, 



0-8 grm 

 02 

 0-02 

 0-01 

 0005 

 01 

 01 

 100 c.c. 



J9H7-5-7-8 {circa) 



Grape sugar. 

 Aqua dest. 



This solution may not be boiled. If desired, it may be sterilised 

 through a Berkefeld filter. 



Fleisch solution is a modified form of Tyrode, and may be made 

 up in two stock solutions, which may be boiled without harm. 



Stock Solution I 



NaCl . 

 KCl . 



Normal NagCOy sol. 

 Aqua dest. . 



Stock Solution II 



MgCla . 



N. HCl sol. . 



N. H3PO4 sol. 



Dextrose 



Aqua dest. . 



Just before use equal parts of the 

 are taken, and Sol. I. is added to Sol. II. The prepared solution 

 (I. and II.) must not be boiled. pH of solution = 7-52. 



two solutions (after cooling) 



