INJECTIONS 239 



with blue litmus paper, and if necessary add more acid till the 

 reaction is decidedly acid. Then add half an ounce of glycerin, 

 two drachms of alcohol, and six drachms of water. We have 

 found this useful, but not so good as the two following. 

 537. Beale's Prussian Blue {How to Work, etc., p. 93). 



Common glycerin .... 1 ounce. 



Spirits of wine 

 Ferrocyanide of potassium . 

 Tincture of perchloride of iron 

 Water .... 



1 „ 



12 grains. 

 1 drachm. 

 4 ounces. 



Dissolve the ferrocyanide in one ounce of the water and glycerin, 

 and add the tincture of iron to another ounce. These solutions 

 should be mixed together very gradually, and well shaken in a 

 bottle, the iron being added to the solution of the ferrocyanide of 

 potassium. Next the spirit and the rest of water are to be added 

 very gradually, the mixture being constantly shaken. 



Injected specimens should be preserved in acidulated glycerin 

 {e.g. with 1 per cent, acetic acid), otherwise the colour may fade. 



538. Beale's Acid Prussian Blue {ibid., p. 296). 



Price's glycerin .... 2 fluid ounces. 



Tinct. of sesquichloride of iron . 10 drops. 



Ferrocyanide of potassium . . 3 grains. 



Strong hydrochloric acid . . 3 drops. 



Water ..... 1 ounce. 



Proceed as before, dissolving the ferrocyanide in one half of 

 the glycerin, the iron in the other, and adding the latter drop 

 by drop to the former. Finally add the water and HCl. Two 

 drachms of alcohol may be added to the whole if desired. 



We find this excellent. 



539. Ranvier's Prussian Blue Glycerin Mass {Traite, p. 120). The 

 Prussian blue fluid, § 537, mixed with one fourth of glycerin. 



540. Thoma's Indigo-Carmine {Arch. Anat. Phys., Anat. Abth., 

 1899, p. 270). Dissolve 0-15 grm. sulphindigotate of soda in 

 50 c.c. water, filter, add 40 c.c. glycerin and gradually, with 

 agitation, 10 c.c. of a filtered 10 per cent, solution of sodium 

 chloride in water. If desired, 3 c.c. of a 1 per cent, solution of 

 morphia may be added to dilate arteries. A fine precipitate is 

 formed, which is injected with the mass. 



541. Gamboge Glycerin (Harting, Das MiJcroskop, 1866, 2, 

 Theil, p. 124). Gamboge rubbed up with water an I added to 

 glycerin ; or a stituratcd alcoholic solution of gamboge added 

 to a mixture of equal parts of glycerin and water. Any excess 

 of alcohol may be got rid of by allowing the mass to stand for 

 twenty-four hours. 



