240 INJECTIONS 



542. Other Colours. Any of the colouring masses, §§ 528 to 532, or 

 other suitable colouring masses, combined with glycerin, either dilute 

 or pure. 



PURELY AQUEOUS MASSES. (See also § 809) 



543. Ranvier's Prussian Blue Aqueous Mass {Traite, p. 120). 

 The soluble Prussian blue, injected without any vehicle. It does 

 not extra vasate. 



544. Muller's Berlin Blue {Arch. mik. Anat., 1865, p. 150). 

 Precipitate a concentrated solution of Berlin blue by means of 

 1^ to 1 volume of 90 per cent, alcohol. The precipitate is very 

 finely divided ; and the fluid may be injected at once. 



545. Mayer's Berlin Blue {Mitth. Zool. Stat. Neapel, 1888, 

 p. 307). A solution of 10 c.c. of tincture of perchloride of iron 

 in 500 c.c. of water is added to a solution of 20 grm. of yellow 

 prussiate of potash in 50 c.c. of water, allowed to stand for 

 twelve hours, decanted, the deposit washed with distilled water 

 on a filter until the washings come through dark blue (one to 

 two days), and the blue dissolved in about a litre of water. It 

 is well to add a little acetic acid and to put up the objects in an 

 acid liquid. 



546. Emery's Aqueous Carmine (ibid., 1881, p. 21). To a 10 per cent, 

 ammoniacal solution of carmine is added acetic acid, with continual 

 stirring, until the colour of the solution changes to blood-red. The 

 supernatant clear solution is injected cold without further preparation. 

 The injected organs are thrown at once into strong alcohol to fix the 

 carmine. For injection of fishes. 



547. Taguchi's Indian Ink {Arch. mik. Anat., 1888, p. 565). 

 Chinese or (better) Japanese ink well rubbed up on a hone until 

 a fluid is obtained that does not run when dropped on thin blotting- 

 paper, nor form a grey ring round the drop. Inject until the 

 preparation appears quite black, and throw it into some hardening 

 liquid (not pure water). 



Della Rosa {Ver. Anat. Ges., 1900, p. 141) recommends the 

 liquid Chinese ink sold in the shops. 



PARTIALLY AQUEOUS MASSES 



548. Joseph's White-of-Egg {Ber. naturw. Sect. Schles. Ges., 

 1879, pp. 36—40 ; Journ. Roy. Mic. Soc, ii, 1882, p. 274). 

 " Filtered white-of-egg, diluted with 1 to 5 per cent, of carmine 

 solution. . . . This mass remains liquid when cold, coagulates in 

 dilute nitric acid, chromic or osmic acid, and remains transparent 

 in the vessels." For invertebrates. 



Grosser {Zeit. zoiss. Mik., xvii, 1900, p. 178) rubs up Indian 

 ink with white-of-egg; Hoffmann {Zeit. Morph. Anthrop., iii, 



