INJECTIONS 241 



1901, p. 2-10) with blood-serum ; so also Hamburger, Zeit, wiss. 

 Mik., XXV, 1908, p. 1 (2 vols, of the ink—" Perltusche "—to 3 of 

 serum). 



549. Bjeloussow's Gum Arabic Mass {Arch. Anat. Phys., 

 1885, p. 379). Make a syrupy solution of gum arable and a 

 saturated solution of borax in water. Mix the solutions in sueh 

 proportions as to have in the mixture 1 part of borax to 2 of 

 gum arable. Kub up the transparent, almost insoluble mass 

 with distilled water, added little by little, then force it tlirough 

 a fine-grained eloth. Repeat these operations until there is 

 obtained a mass that is free from clots. It should then coagulate 

 in the presence of alcohol, undergoing at the same time a dilatation 

 to twice its original volume. The vehicle thus prepared may 

 be combined with any colouring mass except cadmium and 

 cobalt. 



After injection tJie preparation is thrown into alcohol, and the 

 mass sets inniiediately, swelling up as above described and con- 

 sequently showing vessels largely distended. 



Cold-blooded animals may be injected whilst alive with this 

 mass. It does not flow out of cut vessels. Injections keep well 

 in alcohol. If it be desired to remove the mass from any part 

 of a preparation, this is easily done with dilute acetic acid. 



550. Milk has been recently recommended by Fischer (Centralb. 

 (dig. Path., xiii, 1902, p. 277 ; Zeit. wiss. Mik., xx, 1908, p. 224). It 

 runs well, does not extravasate, and can be used for auto-injection of 

 the living subject. 



After injection it should be coagulated by putting the organs for at 

 least twenty-four hours into a mixture of 75 parts of formol, 15 of 

 acetic acid, and 1000 of water (pure formol will not do). They are 

 then sectioned, and the sections stained with Sudan III or Seharlach R, 

 which stain the milk. They cannot be mounted in balsam. 



CELLOIDIN AND OTHER MASSES 



551. Schiefferdecker's Celloidin Masses [Arch. Anat. Phys., 1882 

 [Anat. Abth.], p. 201). (For Corrosion preparations.) See previous 

 editions ; Hochstetter's Modification of Schiefferdecker's Mass 

 {Anat. Anz., 1886, p. 51) ; Budge's Asphaltum Mass {Arch. mik. Anat., 

 xiv, 1877, p. 70), or early editions ; Hover's Shellac Mass {Arch. mik. 

 Anat., 1876, p. 645). F'or this and that of Beli,arminow {Anat. Anz., 

 1888, p. 605), see early editions ; Hover's Oil-colour Masses {Internal. 

 Monatsschr. Anat., 1887, p. 341) ; Severeanu's, Vcrh. Anal. Ges., 21 

 vers, 1906, p. 275 ; Pansch's Starch Mass {Arch. Anal. Entiv., 1877, 

 p. 480; 1880,pp.232, 371 ; 1881, p. 76; 1882, p. 60 ; 1883, p. 265 ; and 

 a modification of the same by Gage, Amer. Mon. Mic. Journ., 1888 

 p. 195) ; Teichmann's Linseed-Oil Masses (S. B. Math. Kl. Krakau 

 Akad., vii, pp. 108, 158 ; Journ. Roy. Mic. Soc, 1882, pp. 125 and 716, 

 and 1895, p. 704) ; Flint's Celluloid (Amer. Journ. Anat., i, 1902, 

 p. 270) ; Huber's (ibid., vi, 1907, p. 39.'}) ; Krassuskaja's Photoxylin 

 {Anal., Heft. 2, xiii, 1904, p. 521). 



