MACERATION CORROSION 245 



563. Sulphocyanides of Ammonium and Potassium (Stiki.inc, Journ. 

 Atidt. (1)1(1 I'liifs., yiyii, 188.*J, p. 208). Ten per cent, solution of either of 

 these salts, for ejiitheliuni. Maeerate small pieces for twenty-four to 

 forty -eight hours. 



Soulier {Tntvaux de Vlnst. 'Aool. de MoutpclUcr, Nouv. Scr., 2, 1891, 

 p. 171) has found that Stiumng's solution greatly deteriorates cellular 

 elements, but that good results are obtained by combining it iviUi a 

 fixing agent. The best results were obtained with a 2 per cent, solution 

 of sulphocyanide combined with licjuid of Ripaut and Petit ; good 

 ones, by combining liquid of Ripaut and Petit with artificial serum of 

 Kronecker instead of sulphocyanide, or with pepsin, eau de Javelle, 

 10 per cent, sulphate of soda, or 1-5 per cent, solution of caustic soda ; 

 also by combining solutions of chloride of sodium, or solutions of 

 caustic potash or soda, with any of the usual fixing agents. 



564. Landois's Solution {Arch. mikr. Anat., 1885, p. 445). 



Saturated sol. of neutral chromate of 



ammonia ..... 5 parts. 



Saturated sol. of phosphate of potash . 5 



Saturated sol. of sulphate of soda . 5 



Distilled water ..... 100 



55 

 5> 



Small pieces of tissue are macerated for one to three, or even 

 four to five days, in the liquid, then brought for twenty-four 

 hours into ammonia carmine diluted with 1 volume of the macera- 

 ting liquid. 



Gierke particularly recommends this liquid for all sorts of 

 macerations, but especially for the central nervous system, for 

 which he finds it superior to all other agents. It is also recom- 

 mended for the same purpose by Nansen (v. Zeit. wiss. Mik., 

 V, 1888, p. 242). 



565. Bichromate of Potash. 0*2 per cent. 



EisiG {Fauna u. Flora Golf. Neapel, 16 Monog., 1887, p. 297) 

 macerates Capitellidic in 0-5 to 1 per cent, solution for months 

 or years, a little thymol being added against mould. 



MUller's Solution, diluted to same strength, or combined with 

 saliva, has also been used. 



Brock (for nervous system of Mollusca, Intern. Monatssch. 

 Anat., i, 1884, p. 349) takes equal parts of 10 per cent, solution 

 of bichromate of potash and visceral fluid of the animal. 



566. Permanganate of Potash is recommended, either alone or 

 combined with alum, as the best dissociating agent for the fibres 

 of the cornea (Rollett, Strieker's Ilandbuch, p. 1108). We have 

 found it, for some objects, very energetic. 



567. Chromic Acid. Generally employed of a strength of 

 about 0-02 per cent. Specially useful for nerve tissues and 

 smooth muscle. Twenty-four hours' maceration will sullice for 

 nerve tissue. About 10 c.c. of the solution should be taken for 

 a cube of 5 millimetres of the tissue (Ranvier). 



