FATTY SUBSTANCES 279 



Arndt, H. J., Verh. dents, path. Ges., xx, 1925, p. 14» ; Brunswik , 

 H., Z. xviss. Mikrosk., xxxix, 1922, p. 316 ; Daddi, L., Arch. ital. Biol., 

 xxvi, 1896, p. 143 ; Froboksk, C. and Sproiinle, G., Z, mikr.-anat. 

 Forsch., xiv, 1928, p. 13 ; Gross, W., Z. xviss. Mikrosk., xlvii, 1930, p. 64 ; 

 Kaufmann, C. and Lkiimann, K,, Zbl. allg. Path., xxxvii, 1926rt, p. 145 ; 

 Arch. path. Anat., cclxi, 1926ft, p. 623 ; Ibid., cclxx, 1928-29, p. 360 ; 

 Z. mikr.-anat. Forsch., xvi, 1929. p. 586 ; Arch. path. Anat., cclxxxiii, 

 1932, p. 190 ; Kay, W. W. and Whitkhkad, R., J. Path. Bact., xxxix, 

 1934, p. 449 ; Ibid., xli, 1935, p. 303 ; Kimmelstikl, P., Zbl. allg. 

 Path., xxxvi, 1925, j). 491 ; Krankheitsforsch., v, 1927, p. 403 ; Larson, 

 H. W., J. Lab. Clin. Med., xviii, 1933, p. 848 ; Laux, F. J., Zbl. allg. 

 Path., xxxviii, 1926, p. 581 ; Leulier, A. and Noel, R., Bull. Histol. 

 appliq., iii, 1926, p. 316 ; Leulier, A. and Revol, L.. ibid., vii. 1930, 

 p. 241 ; LisoN, L., ibid., x, 1933a, p. 237 ; ibid., x, 1933ft, p. 292 ; 

 C. R. Soc. Biol., cxv, 1934, p. 202 ; Arch. Biol. (Liege), xlvi, 1935«, 

 p. 599 ; Ball. Ilistol. appliq., xii, 1935ft, j). 279 ; Histochimie animale, 

 Gauthier-Villars, Paris, 1936 (Part II, Section III, pp. 189-224) ; 

 Llson, L, and Dagnelie, J., Bull. Histol. appliq., xii, 1935, p. 85 ; 

 Mayer, R. M., Krankheitsforsch., vi, 1928, p. 48 ; Romeis, B., Arch, 

 path. Anat., cclxiv, 1927, p. 301 ; Z. mikr.-anat. Forsch., xvi, 1929, 

 p. 525 ; RoMiEU, M., C. R. Soc. Biol., xcii, 1925rt, p. 787 ; C. R. Assoc. 

 Anat., XX, 1925ft, p. 345 ; Schultz, A., Zbl. allg. Path., xxxv, 1924-25, 

 p. 314 ; l^erh. dents, path. Ges., xx, 1925, p. 120 ; Schultz, A. and 

 Lour, G., Zbl. allg. Pa //j.,\ xxxvi, 1925, p. 529 ; Sehrt, E., Histologic 

 und Chemie der Lipoide der zveissen Blutzellen, Thieme, Leipzig, 1927, 

 p. 24 (cited by Froboese and Sprohnle, 1928) ; Steinle, J. V. and 

 Kahlenberg, L., J. Biol. Chem., Ixvii, 1926, p. 425 ; Whitehead, R., 

 J. Path. Bad., xli, 1935, p. 305 ; Yamasaki, K., Fukuoka-Ikivadaigaku- 

 Zasshi, xxiv, 1931, p. 79. 



VARIOUS METHODS AND THEIR RELATIVE MERITS 



650. Stains for Fatty Substances in General. The most widely 

 used method for fatty substances in general is staining with 

 Sudan or Scharlach. Other substances proposed include cyanine, 

 alkanna, chlorophyll, indophenol, extracts of capsicum berries 

 and of carrot ; none of these is in general use and according to 

 Lison (1934) all, with the possible exception of indophenol, are 

 inferior to Sudan and Scharlach. After studying the fat-staining 

 properties of many dyes, Lison (1934) recommended the follow- 

 ing, which he stated were specific for fatty substances : blue 

 B.Z.L. (Ciba). Sudan red B, and Sudan black B (both I. G. 

 Farbenindustrie). 



651. Examination of Vacuoles in Paraffin Sections. Since 

 dehydrating and clearing agents are fat-solvents, fatty substances 

 are represented in paraffin sections as a rule merely by vacuoles. 

 It is unjustifiable to regard the study of such vacuoles as a method 

 for fatty substances for the following reasons : (1) it is irrational 

 to study fatty substances indirectly when direct methods are 

 available. (2) not all vacuoles represent fatty substances, 

 (3) it is impossible to appreciate in vacuolated paraffin sections 

 details that are obvious in sections containing fatty substances, 

 and (4) no test can be applied to vacuoles. 



