GOLGI BODIES ETC. 



307 



toxylin (long method, § 693), {b) stain as for Benda (§ 697), 

 (c) mount unstained for examination of osmicated granulations, 

 {d) stain in safranin and light green (§ 344). For a chart illus- 

 trating Champy-Kull technique, see below. 



Maximow (C R. Soc. Biol., Paris, Ixxix, p. 462) fixes in Champy, 

 washes slightly in water, transfers to mixture of 1 per cent, chromic acid 

 1 part, olacial acetic 2 parts, for twenty-four hours. Wash again for 

 half an hour, place for three daj's in 3 per cent. KjCtjO^. Wash in 



runnmg water. 



Stain sections as above. 



696. Champy-Kull Fixation (Germ-Cells) 



Subsequent 

 method. 



Mount unstained 



(Jhainpy-KuU stain 



Iron ItifmatoxyUn 



Benda Main (ali?ariri 

 and crystal violet) 



Mito- 

 chondria. 



Yellowish. 



Red or 

 pinl<. 



Black. 



Violet. 



Fat. 



Black (extract- 

 able in tur- 

 pentine). 



Black. 



Black, pro- 

 vided it has 

 not been ex- 

 tracted in 

 turpentine. 



Same as above 



Yolk. 



Yellow 

 to black. 



Yellow, 

 to black. 



Same as 

 above. 



Same as 

 above. 



GolRi apparatus. 



Yellowish or does 

 not show. 



Generally will not 

 show in soma- 

 tic cells or 

 ovaries, red in 

 male germ cells. 



Same as above, 

 but black in 

 male germ cells. 



Same as above, 

 but violet in 

 male germ cells. 



Nucleus. 



Yellowish. 



Chromatin blue 

 to greenish (nu- 

 cleolus red). 



Chromatin grey 

 to black (nu- 

 cleolus black) 



Chromatin brown 

 or yellowish 

 (nucleolus vio- 

 let). 



697. Benda's Alizarin Method {Ergebnisse der Anat., xii, 1902 

 (1903), p. 752, and other places) is as follows : — Harden for 

 eight days in strong liquid of Flemming, the acetic acid therein 

 being reduced to 3 drops (or as for Champy-Kull or Regaud). 

 Wash for an hour in water and })ut for twenty-four hours into 

 a mixture of equal parts of pyroligneous acid and 1 per cent, 

 chromic acid, then for twenty-four hours into bichromate of 

 potash of 2 per cent., wash for twenty four hours and imbed 

 in paraffin. Sections on the slide are mordanted for twenty-four 

 hours with 4 per cent, solution of ferric alum or diluted liq. ferri 

 sulfur, oxydat., then rinsed with water and put for twenty-four 

 hours into an amber-yellow aqueous solution of Kahlba.um's 

 sulphalizarinate of soda, prepared by dropping 1 c.c. of saturated 

 alcoholic solution thereof into 80 to 100 c.c. of water. Rinse in 

 water, flood the slides with the solution of cry.stal violet (§ 357) 

 diluted with an equal volume of water, and warm till vapour 

 is given off. Rin.se, differentiate one or two minutes in 30 per 

 cent, acetic acid (till the nuclei come out reddish), wash in running 

 water for five to ten minutes, dry with blotting-paper, dip into 

 absolute alcohol, pass through bergamot oil into xylol and balsam. 



