GOLOI BODIES, ETC. 325 



B. (1) Fix in Bouin-Allen 



^ \ ^ 



(2a) Continue on (2b) Stain for (2c) Use general 



for chromosome and glycogen. histological stains, 



chromatin study. 



C. (1) Fix in Champy or Altmann 



^ i X 



(2a) Continue on (2b) Wash out (2c) Cut frozen 



for mitochondria by thoroughly, proceed sections or tease, 



Altmann or iron to Kolatchew for mount in Farrant 



haematoxylin. Golgi bodies. for fats 



The above is the plan used in this laboratory for getting material 

 from hospitals, abattoirs, and from a distance. Plan A is cheap 

 and needs only the provision of a Winchester of fixative. From 

 it a great deal can be done. Plans B and C are an added help, but 

 not necessary if the preparations in A are successful. So far as 

 cytoplasmic bodies are concerned it is usually possible to make 

 good preparations even after the pieces have been several days, or 

 even a week, in fresh fixatives mentioned. 



732. The Beams' Ultra-Centrifuge in the Study of Cell Inclusions. 

 This is an air-driven centrifuge, consisting of a hollow top with a 

 screw cap. This top spins on a column of air produced by an air 

 pump of the type used for tyre inflating in garages. Recently this 

 centrifuge has become an article of commerce, and our experience 

 with it has convinced us that it is quite indispensable for cytolo- 

 gists and protozoologists. No study of granules in oogenesis, or 

 in such organisms as amoebae or flagellates, can be considered 

 satisfactory unless recourse has been made to material ultra- 

 centrifuged. 



In an experimental ultra-centrifuge made in this laboratory by 

 R. Brown, it was found that even with only two holes in the 

 stator crater, the centrifuge worked well, Gatenby * gives 

 sufficient details for constructing a small ultra-centrifuge, which 

 any good mechanic could turn out on a lathe. The holes in the 

 crater of the stator must be staggered so as to make the rotor 

 revolve. The centrifuge must be placed inside a box, lined with 

 sand for safety, should the rotor disintegrate at high speed. 



733. Parat's Techniques for the "Vacuome." Bhattacharya 

 {Allahabad Univ. Studies, 1927-28) describes Parat's various 

 techniques as foUows : — 



For vital staining, it is essential that a very good quality of neutral 

 red is used. 1/500 neutral red (Krall) in 6/1000 physiological salt 

 solution, in proportion of 1 to 500 volumes respectively, is placed in bath 



* " Biological Laboratory Technique," Churchill, 1937. Centrifuge in 

 neutral salt gum, § 781 and § 1430. 



