326 GOLGI BODIES: ETC. 



at 38° C. for twelve hours or so. The above should be mixed before use 

 with an equal part of Janus green 1/500 in 6/1000 salt solution. Sea 

 water dissolves Janus green equally well. According to Bhattacharya, 

 Janus green by itself is not a good specific either for " Golgi bodies "' or 

 even for the detection of mitochondria, for after a quarter of an hour it 

 becomes harmful and causes vesiculisation and fragmentation of the 

 chondriome. By prolonged action it can colour the " Vacuome " also, 

 and thus confusion between the two structures is caused. Soon after 

 the " vacuome " is coloured by neutral red, Janus green can be intro- 

 duced under the cover slip cautiously. Generally within a quarter of 

 an hour, sometimes after prolonged treatment, mitochondria make their 

 appearance. They do not, however, last long. 

 Fixatives used : — 



(1) Nassonow-Champy modification by Parat and Painleve — 



KgCrgO, . . . . .3 per cent. (2 vols.) 



Acid Chromic . . . . 1 „ (2 vols.) 



Acid Osmic. . . . .2 „ (1 vol.) 



Fix for six to twenty-four hours ; wash in water eighteen to twenty- 

 four hours ; transfer to osmic acid 2 per cent, for twelve days ; sections 

 to be cut 3 — 5 \l thick. Bleaching by Henneguy's method ; staining 

 by Altmann-Kull technique. 



Henneguy's bleaching method is very useful for removing the black- 

 ness of over-osmicated material. It consists in washing slides rapidly 

 before staining in a 1 per cent, aqueous solution of potassium perman- 

 ganate, and afterwards in a 4 per cent, solution of oxalic acid. The 

 bleaching should be controlled under the microscope. Sometimes over- 

 osmication is an advantage, for in certain cases '' Golgi bodies " take 

 on their characteristic black colour long after the fat bodies have been 

 blackened. 



(2) Dietrich after Parat and Bergeot. 



Fix in Regaud's fluid (bichromate-formol) or better still in Helly's 

 fluid for twenty-four hours ; afterwards in a saturated solution of 

 potassivun bichromate at 38° C. in the bath for forty-eight hours ; wash 

 in water for twelve hours ; stain in haematoxylin. 



Haematoxylin in a little alcohol . . . . 1 gr. 



Solution acid acetic 2 per cent, in water. . . 100 c.c. 



Differentiate in potassium ferricyanide solution of Weigert. Accord- 

 ing to Parat this method gives a negative result for " Golgi bodies," for 

 their presence is indicated only by vacuoles, whereas lipoids such as 

 mitochondria and yolk are stained black. 



734. Differentiation between Cell Inclusions. It is frequently 

 somewhat difficult to distinguish between the various categories 

 of cell inclusions. In this section we have provided a series of 

 tables intended to act as a tentative guide to the interpretation 

 of the various images got by representative cytological techniques. 

 These tables are based on work carried out on animals of most 

 orders, hut it would he injudicious for the researcher to depend upon 

 them implicitly, because many exceptions are met with, and the 

 personal factor is to be taken into consideration. The use of such 

 tables, if made with sev^eral methods and in conjunction with a 



