396 BLOOD AND GLANDS 



Dekhuyzen (Anat. Anz., xix, 1901, p. 536) recommends a mixture 

 of either 3 or 9 volumes of '2 per cent, osmic acid with 1 of 6 per cent, 

 acetic acid, containing \ per cent, of methylen blue, which he calls 

 " Osmacet." 



The mercurial liquids of Pasini (§ 458) used to be considered good. 

 Hayem {Du Sunt, etc., Paris, 1889 ; see also Zeit. iviss. Mik., vi, 1889, 

 p. 335) has the following formula : — Sublimate 0-5, salt 1, sulphate of 

 soda 5, and water 200. This should be mixed with blood in the pro- 

 portion of about 1 : 100. Eosin may be added to it. Lowit's formula 

 (Sitzb. k. Akad. Wiss. Wien., xcv, 1887, p. 144) consists of 5 c.c. cold 

 saturated sublimate solution, 5 grm. sulphate of soda, 2 grm. salt, 

 and 300 c.c. water. Mosso finds that both of these are too weak in 

 sublimate. 



DuBOSCQ {Arch. Zool. Exper., vi, 1899, p. 481) uses (for blood of 

 Chilopoda) a solution of acetic acid, copper acetate, copper chloride, 

 osmic acid, thionin, 1 grm. each, water 400, which, mixed with the 

 blood, fixes and stains in about two minutes. 



Formol has lately been used. IVIarcano {Arch, de Med. Exper., xi, 

 1899, p. 434) mixes fresh blood with a mixture of 100 parts of sodium 

 sulphate of sp. gr. 1-020 and 1 of formol ; or with water 85 to 100 parts, 

 sodium chloride 1, and formol 1. 



KizER {Journ. Roy. Mic. Soc, 1900, p. 128) simply mixes 1 drop of 

 blood with 3 of 2 per cent, formalin, and allows to stand for an hour. 



ScHRiDDE {Hcemat. Techn., Jena, 1910, p. 17) lets blood drop into a 

 mixture of 1 part of formol, 9 of liquid of Miiller, and 10 of water, fixes 

 therein for two to four hours at 40° C, filters, washes and brings through 

 alcohol and chloroform into parafiin for sectioning. 



871. Fixing and Preserving of Films. Muir {Journ. of Anat. 

 and Phys., xxvi, 1892) makes cover-glass films and drops them 

 into saturated sublimate solution, and after lialf an hour washes, 

 dehydrates, and passes through xylol into balsam. 



GuLLAND {Brit. Med. Journ., March 13th, 1897 ; Scottish Med. 

 Journ., April, 1899) makes cover-glass films, and after a few 

 seconds drops them face downwards into a solution of : 



Absolute alcohol saturated with eosin . . 25 c.c. 



Pure ether . . . . . . 25 ,, 



Sublimate in absolute alcohol (2 grm. to 10 c.c.) 5 drops. 



After three or four minutes they are washed, stained and 

 mounted in balsam. 



For Jenner's fixing and staining method, see next section. 



Many recent authors fix wet films with formol. Benario 

 {Deut. med. Wochenschr., 1895, p. 572) mixes 1 part of 10 per 

 cent, formol with 9 of alcohol (the mixture must be freshly pre- 

 pared), and plunges films into it for a minute. 



Similarly Gulland, with 1 part of formol to 9 of alcohol. 



Similarly Wermel (see Zeit. zviss. Mik., xvi, 1899, p. 50), 

 who combines various stains (methylen blue, eosin, gentian, etc.) 

 with the formol. 



Edington {Brit. Med. Journ., 1900, p. 19) exposes films for 

 fifteen to thirty minutes to vapour of formol under a bell-jar. 



