398 BLOOD AND GLANDS 



the drop should not be too thick or the dye unevenly spread. 

 Neutral red bodies and mitochondria are stained. 



Sabin {Bull., Johns Hopk. Hosp., 1923, xxxiv, p. 277) uses 

 similar saturated solutions, but adds 0-4 c.c. of the neutral red 

 to 10 c.c. of absolute alcohol and 3 drops of Janus green to every 

 2 c.c. of this solution. 



LiGHTWooD, Hawksley and Bailey {Proc. Roy. Soc. Med., 

 1935, xxviii, p. 405) make a 0-25 per cent, solution of Gurr's 

 vital neutral red chloride, and a 0-4 per cent, solution of Gurr's 

 vital Janus green B. Before use 1-75 c.c, of the neutral red and 

 0-07 c.c. of the Janus green are added to 10 c.c. of absolute 

 alcohol. The slides and coverslips should be perfectly clean. 



(See also Journ. of Exper. Med., 1930, lii, p. 279, and Cun- 

 ningham and ToMKiNS, Folia. Hcemat., 1930, xlii, p. 257.) 



Early observers have used this technique for other dye stuffs. 

 Levaditi {Journ. Phys. Gen., Paris, 1901, p. 245) allows solution 

 of Brillantkresylblmi in alcohol to dry on a slide, puts a drop of 

 blood on the dried layer, and covers. Similarly Cesaris-Demel 

 {Arch. jmth. Anal., 1909, p. 92), with a mixture of this dye and 

 Sudan III ; and Nakanishi {Centralb. Bakt., 1901, p. 98), with 

 methylen blue BB. 



873. Fixed films may be treated with the usual tissue stains, 

 eosin being an important one, as it stains rose-red all parts of 

 blood cells that contain ha;maglobin. Ehrlich's acid haema- 

 toxylin, with 0-5 gr. of eosin dissolved in it, is a good general 

 stain. Or, stain with ha?malum, and then with eosin (0-5 per cent, 

 in alcohol or water). 



Ehrlich's triacid (§ 323) gives good general results, and demon- 

 strates neutrophilous granules. His mixture for eosinophilous 

 cells has been given (§ 339). 



Pappenheim's panoptic triacid (on sale by Griibler) is Ehrlich's 

 triacid with methylen blue in place of the methyl green. 



Chenzinski's inixture, which is good, has been given (§ 341). 

 Stain for six to twenty-four hours in a stove. This gives rise to 

 precipitates. To avoid them (Willebrand, Deutsch. med. 

 Wochenschr., 1901, p. 57) you may make a mixture of equal parts 

 of 0-5 per cent, solution of eosin in 70 per cent, alcohol and 

 saturated solution of methylen blue in water, and add acetic 

 acid of 1 per cent. drojD by drop till the mixture begins to turn 

 red, and filter before use. Or (Michaelis, ibid., 1899, No. 30) 

 make {a) a mixture of 20 parts 1 per cent, aqueous methylen blue 

 with 20 of absolute alcohol, and (6) a mixture of 12 parts 1 per 

 cent, aqueous eosin with 28 of acetone, and for staining mix 

 equal parts of these and stain for half a minute to ten minutes, 



874. Jenner {Lancet, 1899, No. 6, p. 370) mixes equal parts of 

 1-2 to 1-25 per cent, water soluble eosin (Griibler's) and 1 per 

 cent, methylen blue, filters after twenty-four hours, washes the 



