402 BLOOD AND GLANDS 



878. Leishman's Romanowsky Stain {Brit. Med. Journ., 

 March 16th and September 21st, 1901) is as follows : To a 1 per 

 cent, solution of Griibler's medicinal methylen blue in water add 

 0-5 per cent, of sodium carbonate, heat to 65° C. for twelve hours, 

 and let stand for ten days. Then add an equal volume of 0-1 

 per cent, solution of Griibler's eosin extra B, let stand for six to 

 twelve hours, collect the resulting precipitate on a filter, wash it 

 until the wash comes off colourless, dry and powder. The stain 

 is now prepared by the usual makers. For staining, dissolve 

 0-15 grm. in 100 c,c. of pure methyl alcohol. Stain films (air- 

 dried) for one to two minutes ; flood the film with water for 

 eight minutes, and examine or dry (without heat) and mount in 

 xylol balsam. Nuclei in shades of bluish-red, cytoplasm bluish, 

 parasites blue with ruby red chromatin. 



Raadt {Munch, med. Wochenschr., No. 27, 1911 ; Zeit. wiss. 

 Mik., 1912, p. 236) obtains a Romanowsky stain of blood and 

 parasites with Jenner's solution. Films fixed with alcohol and 

 ether are first stained for five to ten minutes in solution of 1 part 

 methylenblau med. puriss. (Hoechst), 0-5 part of lithium carbonate 

 and 100 of water, kept for at least three weeks and diluted with 

 10 volumes of water. Rinse with water, dry with blotting 

 paper, flood with Jenner's solution diluted with 2 or 3 volumes 

 of water, and stain for five to ten minutes. Wash, dry with 

 blotting paper, and mount. See also Scott {Folia Hcem., xii, 

 1911). 



Wright {Journ. Med. Res., vii, 1902, p. 138) describes an 

 essentially similar stain which is largely employed by American 

 workers. The powder which is obtainable from the usual makers 

 is usually dissolved in the proportion of 0-15 grm. to 100 c.c. of 

 pure methyl alcohol. Haden {Journ. Lab. and Clin. Med., Ix, 

 1923, p. 64) recommends that after fixation for one minute in the 

 undiluted stain the staining be carried out for four minutes after 

 dilution with an equal part of buffer solution pH 6-0 to 6-6. He 

 uses Sorensen's phosphate buffer. The slide is then washed in the 

 buffer solution. 



879. Panoptic Staining. Pappenheim {Folia Hcem., 1912, xiii, 

 p. 539) stains films with Giemsa after first fixing and staining with 

 Jenner or May-Griinwald. Air-dried films are fixed for three 

 minutes, after which an equal quantity of distilled water is added 

 and the stain left for one minute. This is poured off and the 

 slide is stained in Giemsa's solution 0-3 c.c. in 10 c.c. of aq. dest., 

 and stained ten to fifteen minutes. This is then washed in 

 distilled water, dried and examined. 



Balint {Klin. Wochenschr., i, 1926, p. 147) fixes in Jenner for 

 five minutes, adds 2 c.c. of buffer solution, and stains for ten 

 minutes. He then washes and stains in Giemsa's diluted 1 drop 

 to 1 c.c. of buffer solution for twentv-five minutes. He finds 



