BLOOD AND GLANDS 403 



that a buffer solution of 6-6 to 7-0 gives the best results, and uses 

 Sorensen's phosphate buffer diluted. Pappeniieim {Anat. Anz., 

 xlii, 1912, p. 325) proceeds as follows in the case of sections. 

 Bring to distilled water and stain for twenty minutes at 35° C. 

 in May-Griinwald or Jenner diluted 1 in 8 of water. The stain 

 is poured off, and the section stained in Giemsa diluted 0-2 e.c. 

 in 15 e.c. of distilled water for forty minutes at 35° C. After a 

 short differentiation of acetic acid, 5 to 6 drops in 100 e.c. aq. dest., 

 it is washed, blotted and dehydrated in equal parts of acetone 

 and absolute alcohol. Then passed into xylol and mounted in 

 cedar wood oil or neutral balsam. Ver)^ good results are obtained 

 with blood-forming organs, granules being well shown. 



Cameron {Journ. of Path, and Bad., xxxv, 1932, p. 933) recom- 

 mends the panoptic method for staining of the granular blood 

 cells of invertebrates. He notes that the staining power of the 

 eosinophil cells is influenced by the jaH of the fixative. At _pH 

 4-3 it is lost, 5-5 difficult to find, at 7-8 they stain distinctly, and 

 from 9 to 10 there is much fusion. 



For a modification of this stain, using methyl green orange, 

 see Kardos {Folia. Ha^tnatol., xii, 1911, p. 39). 



JVIaximow {Ztschr. f. wiss. Mik., xxvi, 1909, p. 177) uses a 

 haematoxylin Azur II. eosin staining for haemapoietic organs 

 which is widely used. Material is fixed in his Zenker formol 

 fixative for six hours at body temperature. Two stock solutions 

 of 1 in 1000 eosin Griibler and 1 in 1000 Azur II. Griibler are made. 

 For staining, 10 e.c. of the eosin solution are diluted with 100 c.e. 

 of distilled water and 10 e.c. of the Azur II. solution added. This 

 is stirred and the sections placed in it immediately and stained 

 for six to twenty-four hours. Differentiate in 96 per cent, alcohol 

 until no more colour comes out, abs. ale, xylol and neutral balsam. 

 The results are almost identical with those obtained using the 

 Giemsa stain. In certain cases it may be necessary to vary the 

 proportions of eosin to Azur II. The stock solutions are good for 

 several months. 



Ugruimow {Ztschr. f. wiss. Mik., xlv, 1928, p. 191) uses stock 

 solutions of Azur II. 1 in 1000 Hollborn and eosin B A extra 

 Hoechst in buffer solution of pH 6-3 to 6-6. Before use these 

 are added to 10 c.e. of distilled water of the proportion of 1-5 to 

 1-6 e.c. eosin to 1 c.e. of Azur II. He stains ten to twelve hours, 

 changing every few hours, and differentiates in acetone, mounting 

 in acid balsam. There are numerous modifications. 



880. Restaining Faded Romanowsky Blood Films. Faded 

 films may be restained with iron alum haematoxylin as follows : 

 Remove coverslip, if used, with warm xylol solution. Place 

 slides in 90 per cent, alcohol overnight. Bring down to water, 

 place in 4 per cent, iron alum for one hour. Wash lightly and 

 transfer to 1 per cent, haematoxylin for three hours. Differentiate 



