BLOOD AND GLANDS 405 



1 to 2 per cent, thionin in water for two to three minutes, then 

 dehydrates in absolute alcohol and counterstains in orange G in 

 oil of cloves, xylol, Canada balsam. 



BuzARD {Bull. (Vhistol. appliq., vii, 1930, p. 264) fixes in either 

 alcohol, formol saline, alcohol formol or bichromate formol. 

 The sections are first stained in 1 per cent, aqueous solution of 

 acid fuchsin for thirty seconds, rinsed rapidly in water and treated 

 with a 0-8 per cent, aqueous solution of bromine for four to five 

 minutes in a closed container until violet. Wash in water, 

 differentiate in 95 per cent, alcohol or 1 per cent, hydrochloric 

 acid in alcohol for one to three minutes until light mauve. Clear 

 in xylol and mount in Canada balsam. After differentiation the 

 nuclei may be stained in iron haematoxylin before dehydration. 

 The period in absolute alcohol should be as short as possible. 

 Granules are carmine red. 



Bolton {Journ. of Morph. and Physiol., liv, 1933, p. 549) uses 

 the ethyl violet Biebrich scarlet stain of Bowie (§ 336) for staining 

 mast cells in fishes after Zenker fixation. Bates {Anal. Rec, 

 Ixi, 1934, p. 231) finds that in mammals watery fixatives are useless 

 and fixes in methyl alcohol followed by Jenner or cresyl violet. 



883. Plasma Cells, Unna's Later Methods. (Unna, in Enzyk. 

 mik. Technik., ii, 1910, p. 411.) 



A. For Large Plasma Cells 



1. Ten minutes in Griibler's polychrome methylen blue 

 solution, wash and drain. Fifteen minutes in 1 per cent, orcein 

 solution (Griibler), without acid ; absolute alcohol, so long as 

 methylen comes away abundantly ; bergamot oil, balsam. 



2. Methylen blue as above, two minutes. Wash well. Then 

 two minutes in glycerin-ether mixture * (Griibler) diluted with 

 4 volumes of water. Wash thoroughly (two to five minutes) ; 

 absolute alcohol, bergamot oil, balsam. 



3. Modification of a method of Pappenheim {Virchow''s Arch., 

 clxiv, 1901, p. 111). Ten minutes in the warm, 20° to 40° C. in 

 Griibler's carbol-pyronin-methyl-green mixture. Cool rapidly, by 

 plunging the recipient containing the tissues into cold water. 

 Remove the tissues with a platinum wire and rinse. Clear and 

 mount in any neutral mounting medium, 



B. For Small Plasma Cells 



4. As No. 2, supra, but only half a minute in the glycerin-ether. 



5. After removal of the celloidin from the section with alcohol 

 and ether, five minutes in polychrome methylen blue, wash, 



* Glycerin-ether CgH^o O3 is a glycerin anhydride. It is a differen- 

 tiating agent for basic dyes. The glycerin-ether mixture in question 

 contains alcohol and glycerin, and can be obtained from Griibler. 



