BLOOD AND GLANDS 411 



As regards the thionin stain, see Hari {Arch. mik. Anat., 

 Iviii, 1901, p. 678). 



Bruno (Bull. Soc. Nat. Napoli, 1905, p. 220) fixes and stains 

 the skin of the frog in a mixture of 100 c.c. of formol of 1-25 per 

 cent, with 8 c.c. of 1 per cent, solution of thionin. Mucous 

 glands red. 



KuLTSCHizKY (Arch. mik. Anat., xlix, 1897, p. 8) fixes in his 

 mixture (§ 60), and stains sections either in safranin with 2 per 

 cent, acetic acid, or in a similar solution of neutral red (two to three 

 days, washing out with alcohol). 



Mayer {Mitt. Zool. Stat. Neapel, xii, 1896, p. 303, or 2nd 

 edition) gives the following two formulae for mixtures that stain 

 exclusively mucus. 



890. Mayer's Mucicarmine {op. cit., last §). One gramme of 

 carmine and 0-5 grm. of aluminium chloride with 2 c.c. of distilled 

 water, heated over a small flame for two minutes, and made up to 

 100 c.c, with 50 per cent, alcohol. This gives a stock solution 

 which is as a rule to be diluted for use tenfold with distilled or tap- 

 water. 



891. Mayer's Muchaematein {ibid.). Haematein 0-2 grm. 

 aluminium chloride 0-1 grm., glycerin 40 c.c, water 60 c.c An 

 alcoholic solution may be made by dissolving in 100 c.c. of 70 

 per cent, alcohol, with or without the additional 2 drops of nitric 

 acid. 



SouTHGATE {Jovm. Path, and Bact.. xxx, 1927, p. 729) proposes 

 the following useful modification in the preparation of muci- 

 carmine. Powdered carmine 1 grm., dry powdered aluminium 

 hydroxide 1 grm. are put into a 500 c.c. flask and 100 c.c. of 50 

 per cent, ethyl alcohol added. Anhydrous aluminium chloride 

 0-5 grm., just powdered, is added, and with frequent shaking the 

 flask is placed on a boiling water-bath and boiled for two and a 

 half minutes exactly. It is then cooled under the tap and filtered. 

 This forms a stock solution good for three months. To stain, 

 dilute 1 in 10, which keeps for about twenty -four hours. Stain 

 fifteen to twenty minutes. The anhydrous aluminium chloride 

 used may be yellow in appearance. 



Sass {Stain Tech., iv, 1929, p. 127) describes a modification in 

 the preparation of Mayer's hsemalum. Dissolve 50 grm. of alum 

 Al2(NH2)2, 4SO4 in 1 litre of boiling water. Remove from hot 

 plate and add 1 grm. haematoxylin. Add 1 grm. sodium iodate 

 (NalOg), cool and filter. Stain should be filtered whenever a 

 metallic scum is present. The solution is best when fresh and 

 retains its properties for six months. The slide is transferred from 

 water to the stain, then washed in distilled water followed by 

 tap-water or sodium carbonate 1 per cent., and again in 

 distilled water. An aqueous or alcoholic counterstain may be 

 used. 



