BLOOD AND GLANDS 413 



minute in 3 })ei- cent. Kg^^'-i^^V' ^^^^^ "^^^' minute in saturated 

 aqueous solution of ha-matoxylin, washing in tap-water eaeli time. 

 The round is repeated once and the slide is differentiated in 

 Weigert's borax ferricyanide (§ 1056). The parietal cells are black. 



Using the tri-colour stain of Bensley (§ 894) he finds that 

 equal parts of a saturated aqueous solution of acid fuchsin and 

 orange G stain the grainilcs of the parietal cells a definite pink in 

 a minute or two, and if followed by a saturated solution of toluidin 

 blue it gives a pretty metachromatic pink in the surface mucus, 

 and in the thick of the cells of the surface and stomach pits. 

 Zymogen and prozymogen are blue. 



Kirk {Amer. Journ. Anal., x, 1910, p. 475), working on the pig 

 stomach, fixes in the Bensley mixture of equal parts 3 per cent. 

 K2Cr207 and saturated solution of HgClg in absolute alcohol 

 (§ 108). He fixes in the dark for half to two hours, after which 

 the tissue is brought through 50 per cent, alcohol and so on to 

 paraffin. He finds that the Bensley tri-colour stain is the best all- 

 round stain. In addition he uses Bensley's neutral gentian and 

 copper chrome hsematoxylin given by Harvey. See also Ferguson 

 {Amer. Journ. Anal., xli, 1928, p. 403), 



Florey and Harding {Journ. Path, and Bad., xl, 1935, p. 212) 

 stain mucus in the surface epithelium of the stomach as follows. 

 Nuclei are first stained at 56° C. for ten minutes in acid carmine. 

 The section is then washed and stained in Ehrlich's anilin oil gentian 

 violet for one minute and washed in Lugol's iodine for one minute. 

 Blot, Differentiate in xylol 4 parts and anilin oil 1 part under the 

 microscope. Blot. Wash in xylol. Rinse in absolute alcohol, 

 xylol. Mucin of superficial cells and goblet cells are an intense blue, 

 Briinner's glands less well stained, 



895. The secretory canaliculi of the parietal cells may be 

 demonstrated by Golgi's bichromate and silver method, especially 

 with rejuvenated material. (See Sacerdotti.) Tissue is left for 

 five to six days in half saturated sulphate of copper, and then 

 for twenty-four hours in osmic-bichromate mixture. Imbed 

 rapidly in paraffin. See R. and L. Monti {Rich. Lab. Anal. 

 Roma, ix, 1902). The indicators neutral red, cyanamin, and 

 naphthol blue give excellent results in the supravital coloration 

 of the contents of the ducts and canaliculi. (Harvey and 

 Bensley, Biol. Bull., xxiii, 1912, p. 239.) 



896. Intestine. Florey and Harding {Journ. Path, and Bad., 

 xl, 1935) use the eosin-aurantia-indulin mixture of Ehrlich (§ 339) 

 for staining of Briinner's glands. Goblet cells are unstained, 

 but Briinner's gland cells and pyloric gland cells are blue-green. 



897. Paneth Cells. The granules of these cells stain well with 

 eosin, iron hiematoxylin, or acid fuchsin after most fixations. 

 Klein {Amer. Journ. Anal., v, 1906, p. 323) working on the 

 guinea-pig fixes in equal parts of alcoholic sublimate and Kopsch's 



