414 BLOOD AND GLANDS 



fluid and stains in Bensley's neutral gentian or in iron hsema- 

 toxylin. Better results are obtained with Bensley's tri-colour 

 stain as follows. Sections are stained one minute in equal parts 

 of a saturated aqueous solution of orange G and acid fuchsin, 

 washed in water, and then stained one minute in a saturated 

 aqueous solution of toluidin blue. Wash in water, absolute 

 alcohol, and mount in balsam : chromatin and prozymogen (basal 

 filaments), intense blue, protoplasm a faint bluish, zymogen red. 

 Goblet cells unstained. See Kull (Arch. mikr. Anat., Ixxvii, 

 1911, p. 541). 



898. Argentophil Cells. Masson {Diagnostics de Laboratoire, 

 1923, p. 701) stains the granules of these cells as follows. Frozen 

 sections of formol fixed material are washed two to three hours in 

 water containing 10 drops of NH3 per litre until all traces of 

 formol or chloride are eliminated. They are placed in stoppered 

 vessels containing Fontana's fluid in the dark for thirty-six to 

 forty hours. Specificity is lost if the time be exceeded. Celloidin 

 or paraffin sections may be treated in the same way , though a 

 longer period in the liquid is usually necessary. The sections are 

 then washed in distilled water, Cajal's toning bath for ten minutes, 

 rinsed in water and washed in hyposulphite solution for one 

 minute. Wash in running water fifteen minutes. In well-stained 

 sections the granules alone appear black. Fontana's liquid is 

 prepared as follows. Ammonia is added drop by drop to a 5 per 

 cent, solution of silver nitrate until the silver oxide formed is 

 just redissolved. More 5 per cent, silver nitrate is now added 

 until there is a permanent opalescence in the liquid which should 

 not smell of ammonia. Cajal's toning bath is as follows. Solu- 

 tion A is 2 per cent, ammonia sulphocyanate in water 3 c.c, 

 NagSgOg 3 grm., water 100 c.c. Solution B is a 1 per cent, solution 

 of gold chloride. The bath is a mixture of equal parts of the two 

 solutions made iminediately before use. 



899. Liver. Ordinary histological and cytological fixatives 

 give excellent results. 



For lattice fibrils see Oppel {Anat. Anz., i, 1890, p. 144 ; vi, 

 1891, p. 168) puts pieces of liver or spleen (alcohol material) for 

 twenty-four hours into a solution of neutral chromate of potash 

 (I to 10 per cent.), then for twenty-four hours into a f per cent, 

 solution of silver nitrate, washes, dehydrates and cuts without 

 imbedding. The lattice fibres are only stained near the surface, 

 so that tangential sections must be made. 



Similarly Berkley {ibid., 1893, p. 772) fixing in picric acid, 

 then in an osmium bichromate mixture, and then silvering. 



The silver methods of Bielschowsky and Rio Hortega (§ 1022) 

 give good results. 



For bile capillaries Beaus {Deutsch. Med. Nat. Ges. Jena, 

 V, 1896, p. 307) uses the rapid method of Golgi hardening in a 



