418 BLOOD AND GLANDS 



changes of distilled water. 7. Mordant for fifteen to thirty minutes 

 (or longer) in 2-5 ^^er cent, aqueous iron alum. 8. Wash in two 

 or three changes of distilled water. 9. Impregnate for a few 

 seconds in diamino silver hydroxide. To 5 c.c. of 10-2 per cent, 

 aqueous silver nitrate solution add strong ammonium hydroxide 

 solution, drop by drop, until the precipitate is just dissolved. 

 Add 5 c.c. of 3-1 per cent, sodium hydroxide to the ammoniated 

 silver solution, redissolve the resultant precipitate with a drop 

 or two of strong ammonium solution and dilute to 50 c.c. with 

 distilled water. 10. Wash briefly in distilled water. 11. Reduce 

 in 10 per cent, aqueous formalin. 12. Wash in water. (If the 

 sections are over-impregnated repeat the process from step 7.) 



13. Tone in 0-2 per cent, yellow gold chloride one to three minutes. 



14. Wash in tap water. 15. Fix in 5 per cent, sodium thio- 

 sulphate five minutes. 16. Wash well in tap water. 17. 

 Dehydrate in 80 per cent, and in 95 per cent, alcohol. 18a. For 

 sections affixed by Wright's method. Complete dehydration in 

 absolute alcohol and dissolve celloidin in equal parts of absolute 

 alcohol and ether. 18b. For celloidin or celloidin sheet sections. 

 Complete dehydration in carbol-xylol (xylol 2 parts, phenol 

 1 part). 19. Clear in xylol. 20. Mount in balsam. 



901. Lymphatic Glands. The methods applicable to this 

 spleen are in use with these glands. For reticular fibres especially, 

 see RoESSLE and Yoshida {Beitr. path. Anal., xlv, 1909, p. 110, 

 or Zeit. wiss. Mik., xxvi, 1909, p. 295). Sections stained with 

 hsematoxylin and eosin, or Weigert's iron haematoxylin, or 

 Bielschowsky's neurofibril stain as applied by Maresch {loc. cit.), 

 § 967. The sections should not remain for more than fifteen to 

 thirty minutes in the oxide bath. 



902. Kidney. Sauer {Arch. mik. Anat., xlvi, 1895, p. 110) 

 finds that for the renal epithelium the best fixative is Carnoy's 

 acetic alcohol with chloroform (three to five hours, washing out 

 with absolute alcohol). A mixture of 9 parts alcohol with 1 of 

 nitric acid is also good, as is the liquid of Perenyi. He stains 

 with iron hsematoxylin, and after-stains in a very weak solution 

 of Saurerubin in 90 per cent, alcohol, which stains the ciliary 

 plateau. He macerates with iodised serum or one-third alcohol, 

 staining afterwards with dahlia. 



McGregor {Amer. Journ. Anat., v, 1929, p. 545), in his study 

 of the glomerulus finds three stains to be the most useful : 

 (a) Heidenhain azan carmine, (b) Ohmori's reinblau-picric acid, 

 (c) Lee-Brown's modification of Mallory's anilin blue. The first of 

 these is a slightly modified form of that given in § 811. The rein- 

 blau-picric acid stain of Oiimori {Virch. Arch., ccxxxiv, 1921, 

 p. 53) is as follows : Nuclei are stained for at least thirty minutes 

 in acid fuchsin or lithium carmine, and then for one minute in 

 reinblau-picric acid. This is prepared by adding a concentrated 



