BLOOD AND GLANDS 419 



watery solution of reinblau to a concentrated watery solution of 

 picric acid until a dark green colour appears. Sections are differ- 

 entiated in absolute alcohol, xylol, balsam. 



Mallory's anilin blue (modified by Lee-Brown, Journ. Urol., 

 xxi, 1929, p. 259) is as follows : Acid fuchsin 1 per cent, for thirty 

 seconds. Distilled water one to two minutes. Mallory's stain : 

 Anilin blue ..... 0-5 grm. ' 

 Orange G . . . . . 2-0 ,, I one to five 



Phosphomolybdic acid . . . 2-0 ,, minutes. 



Distilled water .... 100 c.c. / 

 Distilled water two to five minutes. Phosphomolybdic acid 1 per 

 cent, for thirty seconds. Distilled water one to two minutes. 

 Dehydrate, clear mount. 



Arnold {Anat. Anz., xxi, 1902, p. 417) employs intra-vitam 

 staining methods for the study of the granules of the epithelial 

 cells. Sections of fresh kidney are cut with a Valentin's knife, 

 and brought into a very dilute solution of neutral red, or methylen 

 blue, in which the granules stain in a few minutes or hours. Or 

 saturated solutions of the dyes, or of indigo carmine, may be 

 injected subcutaneously during life, at intervals of fifteen to 

 twenty minutes, and after two to five injections the organ may be 

 excised and sections made and examined (see §§ 739 and 775). 



For demonstration of the membrana propria, see Frisch {Anat. 

 Anz., xlviii, 1915, p. 284). 



For the micro-chemical demonstration of urea, uric acid, and 

 sodium urate, see Schultz {Virch. Arch., cclxxx. 1913, p. 519). 



903. Pancreas. Zymogen granules stain well with eosin, acid 

 fuchsin, or iron hcTmatoxylin after the usual fixatives. Good 

 cytological results are obtained by the methods given in § 684. 

 For the Golgi apparatus, both Da Fano's formalin silver nitrate 

 and the Nassanow modification of the Kolatchew technique 

 {Arch. fur. mikr. Anat., xcvii, 1923, p. 136 ; ciii, 1924, p. 437) 

 are in use. The pancreas stains well supravitally or intravitally 

 with neutral red or Janus green. With the former dye the prozy- 

 mogen granules are stained and later the large Krinom bodies of 

 Chlopin {Arch, fur exp. Zellf, iv, 1927, p. 462) are formed. 

 HiRSCH {Zeit. Zellforsch, xiv, 1932, p. 517 ; xv, p. 37). Duthie 

 {Proc. Roy. Sac, Series B, cxiv, 1933, p. 20). 



904. Methods for Islet Tissue. Bensley {Amer. Journ. Anat., 

 xii, 1911). Animal killed by bleeding ; a cannula introduced 

 into aorta and a solution of neutral red in isotonic salt solution 

 containing 1 in 15,000 neutral red, is injected. Immediately after 

 the pancreas has assumed a faint rosy tint a part of the organ is 

 removed — the islets of Langerhans stain intense yellow-red, and 

 rest faint rosy-pink. In a short time after mounting the islets 

 remain the only stained elements, owing to bleaching in the acini. 

 Method applicable to the counting of the islets of Langerhans. 



14—2 



