BLOOD AND GLANDS 421 



Lane's methods {vide supra), and ordinary haematoxylin and 

 eosin. 



Very good results are obtained after this fixative, using the 

 Cowdry-Bensley acid fuchsin methyl green stain. Bensley's 

 1911 article should be consulted for details of other staining 

 methods. 



Bowie {Anat. Rec, xxix, 1925, p. 57) fixes in HgClg 5 grm., 

 KgCrjOy 2^ grm. Glacial acetic acid 2 c.c. and distilled water 

 100 c.c, made freshly before use. He then stains in a modification 

 of Bensley's neutral gentian in which basic ethyl violet is substi- 

 tuted for gentian violet and biebrich scarlet for orange G, made 

 as follows : Watery solutions of biebrich scarlet (Amer. Aniline 

 Products), and basic ethyl violet (Nat. Aniline Co.) are filtered 

 and mixed in the proportion of one to two. The precipitate is 

 washed in distilled water, dried in warm air, weighed, and a stock 

 solution made in absolute alcohol. Stain for twenty-four hours 

 in a solution of 1 mgm. of the dye in 100 c.c. of 20 per cent, alcohol. 

 Rinse in acetone and differentiate in Bensley's mixture of absolute 

 alcohol 1, oil of cloves 3, controlling under microscope. Staining 

 may be prolonged without harm — ^ cells are blue, j3 cells purple, 

 y cells bright red — different results are obtained if acetic acid be 

 omitted from the fixative. 



Bloom (Anat. Rec, xxxix, 1931, p. 363) fixes in Maximow's 

 Zenker fluid and stains in Mallory's azan stain (§ 961), granules of 

 A cells light red, B cells grey orange and D cells blue. 



See also Babkin, Rubaschkin and Ssawitsch {Arch. f. Mikr. 

 Anat., Bd. 74 ; Helly, ibid., Bd. 67) ; Lane {Amer. Journ. 

 Anat., vii, 1907) ; Saguchi {ibid., vols. 26 and 28) ; Clara 

 (Z. /. mikr. Anat. Forsch, i, 1924, p. 4) ; Kolossow {ibid., 

 xxvii, 1927, p. 43). 



905. Thyroid. Bensley {Amer. Journ. Anat, 1916, xxix) 

 uses brazilin and water blue. Fix gland in Zenkerformol. Sec- 

 tion in paraffin and fix sections to slide with water alone or very 

 little albumen ; pass through toluol, absolute alcohol, water, 

 iodise, and place in this brazilin solution for several hours : — 

 Phosphotungstic acid . . . . 1-0 grm. 



Distilled water ..... 100-0 c.c, 

 Brazilin ...... 0-05 grm. 



The brazilin is first dissolved in a small quantity of distilled 

 water by the aid of heat and added to the phosphotungstic acid 

 solution. This solution goes bad after three days. After staining 

 in the brazilin, wash in water, and jDlace for five minutes in this 

 mixture : — 



Phosphomolybdic acid . . . 1-0 grm, 



Wasserblau . . . . . 0-2 ,, 

 Water 100-0 c.c. 



Wash rapidly in water, dehydrate in absolute alcohol, clear in 



