BLOOD AND GLANDS 423 



dvie to adrenalin. Post chroming brings out the reaction in the 

 former case. 



Cramer {Journ. Physiol., Proc. of Physiol. Soc, lii, 1918, p. 1) 

 fixes the adrenals of rats and mice by suspending them in a wet 

 gauze bag at 37° C. for one and a half hours in a closed tube con- 

 taining osmic acid. The tissue is then transferred to 50 per cent, 

 alcohol and is imbedded in wax. Sections may be mounted 

 directly without fia-ther staining. Both cortical lipoids and 

 adrenalin are blackened by the osmic acid, but the former staining 

 is removed by turps. The author finds that the staining is better 

 than with osmic acid bichromate solutions. 



Ogata and Ogata {Zie^l. Beitr., Ixxi, 1923, p. 376), after 

 fixation in Orths' formol (any formol bichromate fixative will do), 

 stain twenty-four hours in Giemsa (10 drops to 10 c.c. distilled 

 water). After washing this is differentiated in 0-25 per cent, 

 acetic acid and brought through alcohol, xylol to balsam. A 

 modification in the case of frozen sections permits their being 

 dried and blotted and differentiated in acid free acetone, i.e. 

 acetone which has been shaken up with calcium acetate in con- 

 centrated solution. 



HoARE {Amer. Journ. Anat., xlviii, 1931, p. 139) finds that the 

 following mixture is the best fixative : KaCrgO, 2|- grm., HgClg 

 5 grm., and water 100 c.c, to which neutral formol is added 

 immediately before use in the proportion of 1 part formol to 9 of 

 solution. Fixation is carried out for twenty-four hours, after 

 which the pieces are put with various bichromate and osmic 

 hardening mixtures for three to fortj-two days. The original 

 paper slioidd be consulted in view of the large number of methods 

 used. 



Whitehead {Journ. Path, and Bad., xxxv, 1932, p. 415) fixes 

 in 5 per cent. KgCrgO,, to which an equal volume of 10 per cent, 

 formol saline is added after two hours and fixation is carried 

 out for twenty-four hours. He stains in haematoxylin and 

 eosin. 



908. For chromaffin glands in general, see Wistlocki (Bull. 

 Johns Hopkins Hospital, xxxiii, 1922, p. 359), who employs very 

 similar methods. 



The intracellular lipoids may be studied in material fixed in 

 osmic fixatives after paraffin imbedding. Post osmication is 

 desirable. They may be stained in frozen sections by the usual 

 fat stains. 



Deansley {Amer. Journ. Anat., xlix, 1931, p. 475) fixes in 

 Flemming's chrome osmium mixture (§ 47) or uses Ciaccio's 

 lipoid technique. 



Whitehead {Journ. Path, and Bact., xxxix, 1934, p. 443) uses 

 the Schultz' cholesterol reaction (§ 662) for demonstrating this 

 substance in the cortex ; {ibid., xii, 1935, p. 305) he uses the 



