424 BLOOD AND GLANDS 



Sudan IV. fat stain with frozen sections of material fixed in formol 

 saline. 



909. Pituitary. Numerous methods of differential staining have 

 been developed for the anterior lobe cells. Good results are 

 obtainable after most fixatives with Mallory's triple stain or 

 Heidenhain's azan stain. See Bloom {Anat. Rec, xlix, 1930, 

 p. 363) ; Rasmussen {Amer. Journ. Anat., xlvi, 1930, p. 461) ; 

 Colin {C.R. Soc, Biol., Ixxxix, 1923, p. 1229) ; stains in mixtures 

 of acid fuchsin, light green and methyl blue. 



Cleveland and Wolfe {Anat. Rec, li, 1932, p. 409) demon- 

 strate four types of cell as follows. Fix in Regaud's fluid, changing 

 each day, and transfer to 3 per cent. KgCrgO,, changing every 

 twenty-four hours. Wash twenty-four hours and dehydrate 

 slowly, cedar wood oil, xylol, and imbed at 60° C. Stain two to 

 three sections in Ehrlich's hsematoxylin for three minutes and 

 rinse in distilled water. Blue in dilute lithium carbonate and 

 transfer to 5 per cent. KgCrgO,, for three days, changing daily 

 and keeping in the dark. Rinse in distilled water and stain 

 twenty to thirty minutes in 5 per cent, erythrosin. Pass through 

 two changes of distilled water and stain in 2 per cent, orange G 

 in 1 per cent, phosphomolybdic acid for two or three minutes. 

 Rinse and immerse in 1 per cent, anilin blue for thirty to sixty 

 seconds. Rinse, pass through 95 per cent, alcohol and mount. 

 Only Griibler's stains used. See also ibid., v. 1931, p. 409, and 

 Ztschr.fur Zellf., xvii, 1933, p. 420. 



Probably the best and simplest pituitary stain for routine 

 work is that of Biggart {Edinburgh Med. Journ., 1935, p. 42). 

 Fix Zenker-formol eighteen to twenty-four hours. Stain thirty 

 to thirty-five minutes at 50° C. in a mixture of equal parts of 

 0-5 per cent. aq. eosin yellow and 0-3 per cent, pj^rrol blue (isamine 

 blue). Differentiate in the following 5 per cent, sodium carbonate 

 10 parts, absolute alcohol 40 parts. Mount in Gurr's neutral 

 mounting medium or neutral balsam. Acidophil cells red, 

 basophils deep blue, chromophobe cells light blue. 



Crook and Russel {Journ. Path, and Bad., xl, 1935, p. 256) 

 use the following method for differential cell counts after formol 

 saline fixation. Sections are brought to water and are mordanted 

 twelve to eighteen hours in 2-5 per cent. KgCraO, 95 parts, 

 glacial acetic acid 5 parts, washed in running water for two minutes 

 and placed in Lugol iodine for tliree minutes or more. They are 

 decolourised in 95 per cent, alcohol for one hour or more and 

 stained in 1 per cent, acid fuchsin for fifteen minutes. They are 

 then washed in running water for thirty seconds to five minutes, 

 rinsed in distilled water, and counterstained in Mallory's anilin 

 blue mixture for twenty minutes. Lastly, they are washed in 

 running tap water for two to five minutes and are differentiated 

 in 95 per cent, alcohol for twenty seconds to five minutes. Absolute 



