TEGUMENTARY ORGANS 439 



For Trichohyalin, see Gavkzzeni, Monntsschr. prakt. Derm., xlvii, 

 1908, p. 229. 



932. Eleidin. To demonstrate the stratum granulosum and the 

 eleidin granules Ranvier {Arch. Anat. Micr., iii, 1899, p. 1) 

 hardens with alcohol, stains with picro-carmine, and treats with 

 lime-water. The cells swell and show up the granules, which do 

 not change. See loc. cit., other methods for the study of skin. 



Buzzi (see Encycl. mik. Technik., article " Haut ") stains 

 sections for a few minutes in a watch-glassful of water with 2 to 

 3 drops of 1 per cent. Congo red. Similarly Weidenreich, 

 Arch. mik. Anat., Ivii, 1901, p. 583. Other authors recommend 

 nigrosin, or Wasserblau, or orcein. 



See also Joseph, " Dermatohist. Technik," Berlin, 1905, and 

 Dreuw, Med. Klink, Berlin, 1907, Nos. 27 and 28. 



For Cholesterin see Golodetz and Unna, Monatsschr. prakt. 

 Derm., xlvii, 1908, p. 1. 



933. Horn, Hair, Nails and Feathers. The elements of hairs 

 and nails may be isolated by prolonged maceration in 40 per cent, 

 potash solution, or by heating with concentrated sulphuric acid. 

 See also von Nathusius, Zool. Anz., xv, 1892, p. 395. 



Horny tissues stain well in safranin or gentian violet (Reinke, 

 Arch. f. mik. Anat., xxx, 1887, p. 183 ; Ernst, ibid., Ivii, 1896, 

 p. 669 ; Rabl, ibid., xlviii, 1896, p. 489). 



Unna {op. cit. last section, p. 598) stains the tyrosin-bearing 

 keratin in sections of skin for a few seconds or minutes in a 

 mixture of 5 parts of Millon's reagent, 5 of water, and 1 of glycerin, 

 treats shortly with nitric acid of 25 per cent., and mounts in 

 balsam. 



For Espinasse's method see § 177. 



934. Skin-nerves and Nerve-endings. Impregnate with gold 

 chloride. See Chapter XIX, especially § 398. 



935. Tactile Corpuscles. See §§ 398-400. Gold methods are 

 indicated. See also Ranvier, Traite, p. 919 ; Langerhans, 

 Arch. mik. Anat., 1873, p. 730 ; Kultschizky, ibid., 1884, 

 p. 358 ; and Smirnow, Intern. Monatsschr. f. Anat., etc., x, 

 1893, p. 241, who recommends, besides the gold method of Lowit, 

 the rapid bichromate of silver method of Golgi. 



936. Corpuscles of Herbst and Corpuscles of Grandry. Dogiel 

 {Arch. Anat. u. Entwickel., 1891, p. 182) has used the methylen 

 blue method. Four per cent, solution of methylen blue, warmed 

 to 40° C, is injected into blood-vessels of the heads of ducks or 

 geese ; pieces of skin are removed from the beaks, sectioned in 

 pith, and the sections brought on to slides and moistened with 

 aqueous or vitreous humour from the animal and left for ten to 

 thirty minutes exposed to the air, then brought into picrate of 

 ammonia, and treated as described, § 382. Geberg {Intern. 

 Monatsschr. Anat., x, 1893, p. 205) made use of a method of 



