CHAPTER XXXVII 

 MUSCLE AND TENDON (NERVE-ENDINGS) 



STRIATED MUSCLE 



941. Muscle-cells. For these and allied subjects see, inter alia, 

 Behrens, Kossel, und Schiefferdecker, Das Mikroskop, etc., 

 vol. ii, pp. 154-161 ; and Schafer, Proc. Roy. Soc, xlix, 1891, 

 p. 280. 



Iron hsematoxylin gives very fine images of striped muscle, 

 and so does Mallory's phospho-tungstic. 



For dissociation methods see §§ 554, 580. 



To isolate the sarcolemma Solger {Zeit. wiss. Mik., vi, 1889, 

 p. 189) teases fresh muscles in saturated solution of ammonium 

 carbonate. 



942. Nerve-endings — the Methylen Blue Method. For 

 Biedermann's procedure for the muscles of Astacus see § 381 (see 



* also Zeit. wiss. Mik., vi, 1889, p. 65). After impregnating as there 

 directed the carapace should be opened, and the muscles exposed 

 to the air in a roomy moist chamber for from two to six hours. 



For Hydrophilus piceus, Biedermann proceeded by injecting 

 0-5 c.c. of methylen blue solution between the ultimate and pen- 

 ultimate abdominal rings, in the ventral furrow, and keeping 

 the animals alive in 'water for three to four hours, then opened 

 the thorax by two later incisions, and removed the muscles 

 of the first pair of legs and exposed them to the air for ttiree 

 or four hours in a moist chamber, and finally examined in salt 

 solution. 



Gerlach {Sitzb. Akad. Wiss. Miinchen, 1889, ii, p. 125) injected 

 frogs, either through the abdominal vein or through the aorta, 

 with 4 to 5 c.c. of a 1 : 400 solution in 1 per cent, salt solution, 

 and examined pieces of muscle in serum of the animal, after- 

 wards fixing with picrate of ammonia and mounting in glycerin 



jelly. 



The procedure of Dogiel has been given, § 381. 



943. Nerve-endings — the Gold Method. Fischer {Arch. mik. 

 Anat., 1876, p. 865) used the method of LowiT. 



Biedermann (last section) recommends for Astacus a similar 

 procedure, the preliminary treatment with formic acid being 

 omitted, and the muscles being put for a couple of days into 

 glycerin after reduction in the acid. 



Ranvier {Traite, p. 813) finds that for the study of the motor 



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