444 MUSCLE NERVE-ENDINGS 



0'5 per cent, solution of chloride of gold and potassium, and 

 those which become violet are washed and mounted in glycerin. 

 This gives positive images. 



These may also be obtained by putting material fixed by 

 osmic acid into 2 per cent, solution of bichromate of ammonia 

 for a few weeks, then teasing, staining with alum haematoxylin, 

 and mounting in damar. 



Torpedo. Ballowitz {Arch. mik. Anat, xlii, 1893, p. 460) gets 

 the best results by the rapid Golgi impregnation. 



An electric column, with about |- to 1 cm. of tissue round 

 it, is dissected out, and put for three to four days into the osmium 

 bichromate mixture ; then for one to three days into f per cent, 

 silver, cut without imbedding and mounted in xylol balsam. 

 Impregnates all the important elements. See further, on the 

 whole subject, Ballowitz, Enzyk. mik. Techn., 1910, p. 298. 



Cavalie {Bibl. Anat, xiii, 1904, p. 214) takes material fixed 

 with osmic acid of 2 per cent, and impregnates it with gold by 

 the method of Nabias, and mounts in glycerin. 



Raja. IwANZOFF {Bull. Soc. Nat. Moscow, ix, 1895, p. 74) 

 fixes the organ in the tail of Raja with liquid of Flemming, stains 

 with hsemacalcium and eosin, and makes paraffin sections. 



Ballowitz {Anat. Hefte, 1 Abth., vii, 1897, p. 285) finds the 

 method of Golgi excellent for this organ. He also makes sections 

 after fixing in saturated solution of sublimate (in sea-water), or 

 in liquid of Flemming, and examines them in water. Methylen 

 blue may be used, intra-vitam. Gold is little good. 



Gymnotus. Ballowitz {Encycl. mik. Tecknik., p. 303) fixes 

 with Flemming, and makes sections. He also commends impregna- 

 tion with gold chloride, but not the Golgi method. 



Malapterurus. Ballowitz {ibid., p. 202) fixes with picro- 

 sublimate, with Flemming, or with various mixtures of bichro- 

 mate, sublimate, and formol, and uses gold chloride and Golgi 

 impregnations. He macerates in liquid of Miiller or saturated 

 aqueous solution of picric acid. 



TENDON 



948. Tendons. Retterer (C. R. Soc. Biol., x, 1898, p. 580) 

 fixes in equal parts of saturated solutions of sublimate and picric 

 acid, puts for one to three days into saturated picric acid with 

 2 to 3 per cent, of sodium chloride, to remove the mucin, and 

 imbeds in paraffin. 



949. Union of Muscle and Tendon. For this see Retterer and 

 Lelievre, C. R. Soc. Biol., 1911, No. 12 (orcein for twenty-four 

 hours, followed by iron hsematoxylin) ; and Schultze ( Verh. 

 phys. med. Ges. Wiirzburg, 1911, p. 33) treats for a day or two 

 with a mixture of equal parts of 2 per cent, bichromate of potash 



