MUSCLE NERVE-ENDINGS 445 



and alcohol, in the dark, then for two days with 0-5 per cent, 

 solution of haematoxylin in alcohol of 70 per cent., then with 

 Van Gieson's picro-siiurefuchsin). 



950. Corpuscles of Golgi (Ranvier, Traite, p. 929). Take the 

 tendon of the anterior and superior insertion of the gemini muscles 

 of the rabbit. Treat it by the formic acid and gold method 

 (§ 404), and after reduction scrape with a scalpel, in order to 

 remove the muscle-fibres that mask the musculo-tendinous 

 organs. 



Marchi's methods for the tendons of the motores bulbi oculi 

 {Archivio per le Scienze Mediche, v, No. 15). The enucleated eyes, 

 together with their muscles, were put for not less than three 

 days into 2 per cent, bichromate of potash. The muscles and 

 tendons were then carefully dissected out, stained with gold 

 chloride and osmic acid (Golgi's method), and by the method 

 of Manfredi, § 407. Mount in glycerin. The methods only 

 succeed completely during fine, sunny weather. 



RuFFiNi {Atti R. Ace. Lineei Roma Tend. [5], i, 1892, p. 442) 

 recommends the method of Fischer. 



CiACCio {Mem. R. Aec. Sci. Bologna [4], t. x, 1890, p. 301) 

 puts tendons of Amphibia into 0-1 per cent, hydrochloric acid 

 or 0-2 per cent, acetic acid until transparent ; then for five 

 minutes into a mixture of 0-1 per cent, gold chloride and 0-1 

 per cent, potassium chloride ; then back into the acetic acid, 

 for a day in the dark, and for two or three hours more in the 

 sunlight. When they have become somewhat violet they are 

 put for a day into 0-1 per cent, osmic acid, and finally mounted 

 in glycerin acidulated with 0-5 per cent, of acetic or formic acid. 



DoGiEL {Arch. mik. Anat., Ixvii, 1906, p. 638) stretches tendons 

 of eye-muscles on cardboard with hedgehog spines, puts for four 

 or five days into nitrate of silver of 1 to 2 per cent., reduces for a 

 day in pyrogallic acid with formol, and imbeds in celloidin. 



SMOOTH MUSCLE 



951. Tests for Smooth Muscle. Picro-saurefuchsin, § 280, 

 stains muscle yellow, connective tissue red. 



Picro-nigrosin, § 353, stains muscle yellowish, connective tissue 

 blue. 



Unna {Eneycl. mik. Technik., article " Kollagen ") stains for 

 twenty-four hours in orcein 1 part, Wasserblau 0-25, alcohol 60, 

 glycerin 10, water 30, which gives muscle in a mixed tone, collagen 

 blue, elastin reddish. See also a complicated process with 

 methylen blue in Monatssch. prakt. Dermatol., xix, 1894, p. 533, 

 and another with orcein, haematein, saurefuchsin and picric 

 acid. 



Retterer (C. R. Soc. Biol., 1887, p. 645) fixes in 10 volumes 



