NISSL. GRANULES 471 



through the ascending series of alcohols, cajeput oil. and xylol 

 into balsam. The preparations keep better than those stained 

 with thionin or toluidin blue, and are particularly useful for 

 photographic piu'poses and cyto-architectural studies. The cresyl 

 violet R.R. can also be used in 0-5 to 1 percent, watery solutions ; 

 paraffin sections stuck to slides are well stained after some hours, 

 when they are differentiated in alcohol and mounted in the usual 

 way. 



Rehm (Munch, med. Wochenschr., xxxix, 1892, p. 217) floats sections 

 for half a minute to a minute on a hot 01 per cent, solution of methylen 

 blue, differentiates them in 96 per cent, alcohol, and clears them with 

 origanum oil. 



LuGAito {Rev. Neurol, and Psych., ill, 1905, p. 339) fixes for forty- 

 eight hours in 5 per cent, nitric acid in absolute alcohol, imbeds in 

 paraffin, and stains sections for several hours stuck to slides in 1 : 2000 

 or 1 : 3000 toluidin blue ; after a quick wash the stain is fixed by 

 means of a 4 per cent, solution of ammonium molybdate ; wash, 

 dehydrate, clear in xylol, and mount in balsam. 



Lkniiossek {Fein. Ban d. Nervens., 1895) stains sections from formol 

 material in a concentrated watery solution of thionin, rinses them with 

 water, and mounts them with Nissl. 



JuLiusBURGER {NeuTol. Ccntrbl., xvi, 1897, p. 259) stains sections 

 of materials fixed in Orth's fluid and imbedded in celloidin, either with 

 Nissl's methylen blue or with warmed neutral red. 



Rosin {Deutsche med. Wochenschr., xxiv, 1898, p. 615) prefers neutral 

 red for sections of material fixed in formalin. 



Leniiossek {Neurol. Centrbl., xvii, 1898, p. 577) fixes ganglia either 

 in Carnoy's fluid or in equal parts of a concentrated solution of corrosive 

 sublimate and a saturated watery solution of picric acid. He imbeds 

 either in paraifin or celloidin and stains the sections in a concentrated 

 watery solution of toluidin blue overnight, rinses with water, differen- 

 tiates quickly with alcohol, and clears with xylol or-carbol xylol. 



Van Gehuchten and Nelis {La Cellule, xiv, 1898, p. 374) recommend 

 fixing spinal ganglia in Gilson's mixture, and staining thin paraffin 

 sections in a watery solution of toluidin blue. 



Van Gehlx'uten uses paraffin sections mounted on slides by the 

 water method, and stains them for five to six hours in Nissl's methylen 

 blue solution in the incubator at 35° or 40° C. 



GoTUARD (C R. Soc. Biol., v, 1898, p. 330) stains celloidin sections 

 for twenty-four hours in Unna's polychrome methylen blue, and 

 differentiates them with a mixture of 5 parts of creosote, 4 of cajeput 

 oil, 5 to 8 of xylol, and 16 of absolute alcohol. The mixture is removed 

 with absolute alcohol and sections mounted in dammar after clearintr 

 with cajeput oil. 



LuiTHLEN and Sorgo {Neurol. Centrbl., xvii, 1898, p. 640) differentiate 

 in Unna's glycerol-ether mixture after staining with polychrome 

 methylen blue. They remove the differentiating mixture with absolute 

 alcohol and clear with origanum oil. 



Similarly Lennhoff {ibid., 1910, p. 20) ; or, polychrome methylen 

 blue two minutes, distilled water (juickly, carbol-pyronin-methyl 

 green twenty minutes ; distilled water quickly, absolute alcohol, oil, 

 balsam. 



Lord {Journ. Ment. Sc, xliv.. 1898, p. 693) makes sections from frozen 

 fresh tissues, treats them for a few seconds with a mixture of equal 

 parts of 6 per cent, formaldehyde and saturated solution of picric acid. 



