SHADOW AUTOR ADIOG K A PHY 



2. Boyd, G. A., "Autoradiography in biology 



and medicine", Academic Press, New York, 

 1955. 



3. DocKUM, N. L., Coleman, E. J., and Vogt, 



G. S., "Detection of plutonium contami- 

 nation in humans by the autoradiographic 

 method". Stain Technology, 33(3), 137-142 

 (1958). 



4. DocKUM, N. L., AND Case, A. C, "Autoradio- 



graphic analysis of plutonium deposition in 

 human skin", Acta Radiologica, 50, 559-64 

 (1958). 



5. Marks, S., Dockum, N. L., and Bustad, L. 



K., "Histopathology of th3'roid gland of 

 sheep in prolonged administration of 1"^", 

 Am. J. Path., 33, 219-250 (1957). 



6. Dockum, N. L., and Healy, J. W., "Spot 



diameter method of quantitative auto- 

 radiography of ruthenium^o^ particles in 

 lung tissue". Stain Technology, 32(5), 209- 

 213 (1957). 



7. Unpublished data, W. J. Bair. 



8. Vogt, G. and Kawin, B., "Localization of 



radioelements in rat ovary". Document 

 HW-53500, p. 120-123 (Unclassified), 1957. 



9. GuiDOTTi, G. AND Levi Setti, R., "Auto- 



radiography of tracks from beta particle 

 emitters in tissues". Stain Technology, 31, 

 57-65 (1956). 



10. Unpublished data, N. L. Dockum. 



11. Hungate, F. p., Uhler, R. L., Cline, J. F. 



AND Stewart, J. D., "Decontamination 

 of plants exposed to a simulated reactor 

 burn", Document HW-63173 (Unclassified), 

 1959. 



12. Jee, Webster S. S., Arnold, J. S., Mical, 



R., Lowe, M., Bird, B. and Twente, J. A. 

 "The sequence of histopathologic bone 

 changes in bones containing plutonium", p. 

 148-189. Univ. of Utah Radiobiology Lab- 

 oratory Annual Progress Report, COO-218, 

 1959. 



13. O'Brien, R. T., and George, L. A. II, "Prep- 



aration of autoradiograms for electron mi- 

 croscopy". Nature, 183, 1461-1462 (1959). 



14. Unpublished data, Borasky, R. and Dockum, 



N. L. 



15. George, L. A. II, and Vogt, G. S., "Electron 



microscopy of autoradiographed radioactive 

 particles", Nature, 184, 1474-1475 (1959). 



16. Dockum, N. L., Vogt, G. S., and Coleman, 



E. J., "Applications of autoradiography 

 in biological research", J. Biol. Phot. 

 A,s.soc.,27, 1-18 (1959). 



Norman L. Dockum 



SHADOW AUTORADIOGRAPHY 



Shadow autoradiography is a technique 

 that enables one to differentiate between 

 radioactive and non-radioactive particles. 

 The source of the particles may be suspen- 

 sions or aerosols. The technique is described 

 below. 



Droplets of particle suspensions are placed 

 on clean glass microscope slides, spread and 

 allowed to dry. Aerosol particles may be 

 collected directly on the glass substrate by 

 gravity or by impaction methods. If the par- 

 ticles from aerosols are collected on mem- 

 brane filters, the membrane filter is dis- 

 solved in a suitable volume of acetone and 



(a) 



(b) 

 Fig. 1. Alpha emitters. Photomicrographs of 

 chrome-shadowed particles before (a) and after 

 (b) autoradiography. Alpha-emitting radioactive 

 particles are those surrounded by star clusters of 

 alpha tracks. (Courtesy N. L. Dockum). 



11 



