i 



I 



BLOOD 



[Z~\ PARTICLES FROM AEROSOL WITH Sr^SO^ 

 ^^ PARTICLES FROM AEROSOL WITH Ru'^^Oj 

 {■ PARTICLES FROM AEROSOL WITH Pu^'^O, 



1 



050 060 070 

 SIZE IN MICRONS 



Fig. 8. Particle size distribution 



though the size range and mean size for the 

 particles from the different aerosols are 

 similar, there are striking differences in 

 shape and aggregation tendencies. 



REFERENCES 



1. KuMAi.MoTOi, "Encyclopedia of Microscopj^," 



1961. 



2. BORASKY, R., AND Mastel, B., AEC R + D 



Report, No. H.W. 46722 General Electric Co., 

 Richland, Washington, 1956. 



3. Fitzgerald, J. J. and Detwiler, C. G., 



KAPL-1088, General Electric Co., Schenec- 

 tady, New York, 1954. 



R. BORASKY 



BLOOD* 



This method is designed to involve the 

 least possible technical manipulation of the 

 blood sample both before and after fixation. 

 The sample was centrifuged to concentrate 

 the buffy coat, thereby obtaining minimal 

 contamination by erythrocytes. No antico- 

 agulant or any other foreign substance was 

 added to the blood prior to fixation. 



About 6-7cc of blood was obtained by 

 venipuncture either (a) by withdrawal with 

 a lOcc syringe fitted with a 20-gauge needle 



* (Excerpt of fixation, preparation, obtaining 

 of, and microscopy of specimens taken from "Elec- 

 tron Microscopic Atlas of Normal and Leukemic 

 Human Blood") 



and transference to a lOcc Lusteroid centri- 

 fuge tube (International), precooled to 5-10° 

 C; or (b) by needle drip directly into the 

 tube. The syringe had been previously 

 silicon-coated with Dow-Corning 200, 2 per- 

 cent in ecu , by immersion and baking for 

 3^-1 hour at 450-550° C. The needle had 

 been coated with 10 percent aqueous Armour 

 Monocote [tris-(2-hydroxyethyldodecyl)- 

 NH4CI] by immersion, draining, and air 

 drying. The ice-cooled sample was then 

 centrifuged at 1500 rpm for 15 minutes at 

 0° C (relative centrifugal force — 265; Inter- 

 national model PR-2, refrigerated, angle 

 head centrifuge). The buffj- coat was aspi- 

 rated with a silicon-coated pipette and trans- 

 ferred to a glass tube containing 5cc of 1 

 percent Veronal-buffered (pH — 7.4) OSO4 at 

 5-10° C. It was fixed for }^-l hour, usually 

 the former. Between the successive steps 

 (3-^-1 horn-) of fixation, dehydration, and 

 methacrylate infiltration, the specimen was 

 centrifuged for 1-lH minutes at 1500 rpm 

 (relative centrifugal force — 385; Claj^-Adams 

 Safeguard centrifuge) in glass tubes (alcohol 

 dissolves Lusteroid!). After each centrifuga- 

 tion the supernatant fluid was decanted, the 

 next fluid added, and the tube manually 

 agitated to produce a suspension. The last 

 methacrylate suspension (6 parts n-butyl, 1 

 part methyl) was permitted to settle by 

 gravity in 00 gelatin capsules for 3'^-l hour 



77 



