ELECTRON MICROSCOPY 



V " *-vt fit -■• 



■Hi .-i^il^^ 



M- 





«« 



J5 







Neutrophilic Promyelocyte 

 Fig. 3. (a) Cell of granulocytic leukemia 







V 

 ^ 





E:!^^ aitj* 



Neutrophilic metamyelocyte 

 Fig. 3. (b) Cell of granulocytic leukemia 





Neutrophilic myelocyte 

 Fig. 3. (c) Cell of granulocytic leukemia 



to avoid close packing. This also eliminated 

 bubble formation during polymerization, 

 which was performed overnight at 47° C 

 with dry heat in an oven. Sections were cut 

 on a Porter-Blum ultramicrotome using a 

 glass knife and were mounted on copper 

 grids covered by Formvar or carbon mem- 

 branes. Three RCA electron microscopes 

 were used for viewing and photography — an 

 EMU-2, an EML-IB, and an EMU-3. The 

 micrographs were taken on 2 by 10 inch or 

 3M by 4 inch Kodak lantern-slide medium 

 plates and were printed by projection en- 

 largement. Neither the negatives nor prints 

 were retouched. 



James A. Freeman 



BOTANICAL APPLICATIONS 



Introduction. The high resolution ob- 

 tainable with the electron microscope per- 

 mits it to be used to great advantage for a 

 mmiber of different problems in botanical 



80 



