FLYING SPOT MICROSCOPY 



DELAYED 



PULSe 

 GENERATOR 



X 



VERTICAL 

 SAWTOOTH 

 GENERATOr? 



TIMING 



PULSE 



GENERATOR 



HORIZONTAL 

 SAWTOOTH 

 GENERATOR 



INTERVAL 

 GATE 



INTERVAL 

 COUNTER 



1 



DELAYED 



PULSE 

 GENERATOR 



VERTICAL 

 SAWTOOTH 

 AMPLIFIER 



HORIZONTAL 

 SAWTOOTH 

 AMPLIFIER 



EXPOSURE 

 GATE 



EXPOSURE 

 COUNTER 



COINCIDENCE 

 GATE 



CAMERA 

 PULL-DOWN 



PULSE 

 AMPLIFIER 



FIRST SURFACE 

 MIRROR 



1/ 



wv 



U.V. 



SCANNER 



U8E 



REFLECTING I 

 MICROSCOPE 



MONITOR 

 TUBE 



16 MM 



cine' 



CAMERA 



Fig. 



1. Variable sweep -speed UV flying-spot TV microscope 



by means of the delaj^ circuit and by vary- 

 ing the pulse \vidths, the spot size may be 

 varied down to one picture element. Figure 

 3 shows the operation of these circuits. The 

 drawings in this Figure lettered A and B 

 show the individual effect of the pulses and 

 their combined effect is showm on the face of 

 the scanner tube. 



A recent modification of the equipment 

 now allows simultaneous viewing of the 

 specimen in visible and ultraviolet Hght. A 

 visible light tube has been mounted on the 

 equipment at 90° to the ultraviolet tube. 

 A first-surface mirror and beam-spUtter ar- 

 rangement allow the two outputs to be com- 

 bined optically. 



To view a specimen simultaneously in 

 both wavelengths of hght, opposite halves of 

 each raster are masked off and the remaining 

 output is brought into optical registration. 

 The first half of the horizontal scan, in the 

 focal plane, is then ultraviolet and the second 

 half is visible light, and the two scan the 

 same area of the specimen. The monitor tube 

 then displays the two images in a side-by- 

 side presentation. This arrangement is shown 

 in Figure 4. 



With only the ultraviolet tube available, 

 positioning the intensified spot required that 

 the background intensit}' be brought up to a 

 level which would allow viewing of the entire 

 field. This would require increasing the video 

 gain to a level that would cause overloading 

 of the A'ideo amplifier in the intensified area 



Fig. 2. Arrangement of equipment. 



335 



