MEDICO-BIOLOGIC RESEARCH 



ing visualization of microstructures is very 

 similar to that in histology. No one, how- 

 ever, calls histological specimen "shadow" 

 of the dyes used for their visualization. In 

 order to avoid this misunderstanding we do 

 not use the term "x-ray shadow" in our 

 work, but call any radiograph of a structure 

 an "x-ray image" of this. Resink (98) calls 

 a real x-ray image of a real structure "supra- 

 liminae" contrary to "infraliminae" or 

 unreal image caused by superimposition of 

 images of other structures or by pemmibras 

 of other origin. If the microradiograph is 

 made in accordance with the above rules its 

 images will be mostly "supraliminae." 



However, real microradiographic images 

 are not always easy to interpret; they have 

 too many peculiarities characteristic only 

 for x-ray images. 



The student of morphology as it is seen 

 in microradiographs is able to compare 

 radiographical images with known anatom- 



ical and histological patterns and to make 

 the three-dimensional reconstruction of 

 microstructures from their images in serial 

 microradiographs (81) (82). It is necessary 

 to emphasize the important works of Vincent 

 (111) (112) (113) (145), Lacroix and Ponlot 

 (79), who were the first to use microradiog- 

 raphy, autoradiography and histological 

 coloring of the same section of bone (bone 

 was decalcified only for histology). 



Bohatirchuk (30) proposed "stain histo- 

 radiography" for simultaneous study of un- 

 decalcified bone together with surrounding 

 soft tissues and bone marrow (unfortu- 

 nately, it was impossible to call this method 

 "color microradiography" because of pos- 

 sible misunderstanding). In this method a 

 section of undecalcified bone, usually 10 n 



o 



thick, is radiographed with x-rays 1.0 A 

 long and subjected afterward to coloring 

 procedures without previous decalcification 

 or disembedding (Fig. 12). 



A 



Fig. 12. A — MRD, B — Stained specimen of the same human bone, 72, lOyu, approx. X256. The typical 

 stain-historadiograph. The presence of decalcified tissue with cells is shown in those parts of specimen 

 which do not reveal any calcium. Especially conspicuous this decalcified tissue is in those parts which 

 are in close contact with the calcium-containing bone (dark black in microphotograph). 



607 



